Recombinant eIF4A1 Antibody - 1차 항체, specific to EIF4A1, Rabbit IgG

Cat. No.: Ab101344

GRADE & PURITY Recombinant, Validated, ExactAb™, 1.3 mg/mL

Application

WB

Host species

토끼

Reactive species

마우스, 인간, 조언

Isotype

Rabbit IgG

Conjugation

Unconjugated

개요

Rabbit anti Human eIF4A1 Antibody, Recombinant, could be used for WB and so on.

Application:

WB: 1/500-1/1000

Protein Function

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Specifications

Product Name

Recombinant eIF4A1 Antibody - 1차 항체, specific to EIF4A1, Rabbit IgG

동의어

ATP-dependent RNA helicase eIF4A-1 | DDX2 | DDX2A | eIF 4A I | eIF-4A-I | EIF4A | eIF4A I | eIF4A-I | EIF4A1 | Eukaryotic initiation factor 4A-I | Eukaryotic initiation factor 4AI | eukaryotic translation initiation factor 4A, isoform 1 | Eukaryotic trans

사양 및 순도

ExactAb™, Validated, Recombinant, 1.3 mg/mL

숙주 종

토끼

특이성

EIF4A1

면역원

A synthetic peptide derived from human eIF4A1 (AA 301-314)

포지티브 제어

WB: HeLa, K562, MCF7, Jurkat, Hep G2, HEK293, NIH/3T3 and C6 cell lysates.

변화

Unconjugated

등급

ExactAb™, Recombinant, Validated

아이소타입

Rabbit IgG

SDS-PAGE

150 kDa

정화 방법

Antigen affinity purified

집중력

1.3 mg/mL

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: Recombinant eIF4A1 Antibody (Ab101344)

Recombinant eIF4A1 Antibody (Ab101344) - Western Blot
All lanes: Recombinant eIF4A1 Antibody (Ab101344) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 46 kDa
Observed band size: 45 kDa
Exposure time: 188.9 s

적용

• 적용: Dilution info
• WB:

  1/500-1/1000

관련 대상(인간)

EIF4A1 Tchem Eukaryotic initiation factor 4A-I (0 Activities)

Discover Target: EIF4A1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

작용 메커니즘

자주 묻는 질문

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant eIF4A1 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.