Recombinant eIF4B Antibody - 1차 항체, specific to EIF4B, Rabbit IgG

Cat. No.: Ab101365

GRADE & PURITY Recombinant, Validated, ExactAb™, 0.2 mg/mL

Application

IF/ICC, IHC, WB

Host species

토끼

Reactive species

인간

Isotype

Rabbit IgG

Conjugation

Unconjugated

개요

Rabbit anti Human eIF4B Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

Specifications

Product Name

Recombinant eIF4B Antibody - 1차 항체, specific to EIF4B, Rabbit IgG

동의어

Eif4a2 | eIF4B | EIF-4B | eukaryotic translation initiation factor 4B | PRO1843 | 2310046H11Rik antibody | AL024095 antibody | C85189 antibody | EIF 4B antibody | eIF-4B antibody | EIF4B antibody | Eukaryotic initiation factor 4B antibody | Eukaryotic tra

사양 및 순도

ExactAb™, Validated, Recombinant, 0.2 mg/mL

숙주 종

토끼

특이성

EIF4B

면역원

A synthetic peptide derived from human eIF4B (AA 598-611)

포지티브 제어

WB: HeLa, U2OS and A549 cell lysates. IHC: Human lung cancer tissue. IF/ICC: HeLa cells.

변화

Unconjugated

등급

ExactAb™, Recombinant, Validated

아이소타입

Rabbit IgG

SDS-PAGE

150 kDa

정화 방법

Antigen affinity purified

집중력

0.2 mg/mL

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: Recombinant eIF4B Antibody (Ab101365)

Recombinant eIF4B Antibody (Ab101365) - Western Blot
All lanes: Recombinant eIF4B Antibody (Ab101365) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 69 kDa
Observed band size: 78 kDa
Exposure time: 10.0 s

IHC: Recombinant eIF4B Antibody (Ab101365)

Recombinant eIF4B Antibody (Ab101365) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant eIF4B Antibody (Ab101365). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: Recombinant eIF4B Antibody (Ab101365)

Recombinant eIF4B Antibody (Ab101365) - IF/ICC
IF analysis of Recombinant eIF4B Antibody (Ab101365) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant eIF4B Antibody (Ab101365) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

적용

• 적용: Dilution info
• WB:

  1/500-1/1000

• IHC:

  1/50 - 1/100;

• IF/ICC:

  1/200;

관련 대상(인간)

EIF4B Tbio Eukaryotic translation initiation factor 4B (0 Activities)

Discover Target: EIF4B

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

작용 메커니즘

자주 묻는 질문

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant eIF4B Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.