GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
ExactAb™, Validated, Recombinant, High Performance, See COA ExactAb™,High Performance,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
개요
Rabbit anti Human eIF5A Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Specifications
Product Name
Recombinant eIF5A Antibody - 1차 항체, specific to eIF5A
ExactAb™, Validated, Recombinant, High Performance, See COA
생화학 및 생리적 메커니즘
mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved
숙주 종
토끼
특이성
eIF5A
면역원
A synthetic peptide derived from human eIF5A1 (AA 1-47).
포지티브 제어
WB: HeLa, Jurkat, MCF7, Raji, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Human uterus cancer tissue. IF/ICC: HeLa cells.
변화
Unconjugated
등급
ExactAb™, High Performance, Recombinant, Validated
라이트 체인 유형
kappa
SDS-PAGE
150 kDa
정화 방법
Protein A purified
집중력
See COA
Product Line
biological_products
보관 및 배송
모양
Liquid
보관 조건
Store at -20°C,Avoid repeated freezing and thawing
배송
Ice chest + Ice pads
안정성 및 스토리지
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
이미지
Recombinant eIF5A Antibody (Ab101422) - Western Blot All lanes: Recombinant eIF5A Antibody (Ab101422) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 17 kDa Observed band size: 15 kDa Exposure time: 7.5 s
Recombinant eIF5A Antibody (Ab101422) - IHC Immunohistochemical analysis of paraffin-embedded human uterus cancer, using Recombinant eIF5A Antibody (Ab101422).
Recombinant eIF5A Antibody (Ab101422) - IF/ICC Immunofluorescent analysis of HeLa cells, using the Recombinant eIF5A Antibody (Ab101422) at 1/50 dilution.
Recombinant eIF5A Antibody (Ab101422) - IF/ICC Immunofluorescent analysis of HeLa cells, using the Recombinant eIF5A Antibody (Ab101422) at 1/50 dilution.
적용
적용
Dilution info
IHC
1/100 - 1/200
IF/ICC
1/50-1/200
WB
1/1000 - 1/5000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
리뷰
고객 리뷰
자주 묻는 질문
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Recombinant mean?
Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant eIF5A Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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