등급 & 순도Animal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.≥95%(SDS-PAGE)
Accession #
Q13641-1
Protein Tag
C-His
Expression system
HEK293
Endotoxin Concentration
<0.1 EU/μg
Bioactivity
Immobilized Human TPBG at 1 μg/mL (100 μL/well) can bind TPBG Antibody with a linear range of 0.1-2 ng/mL
Animal Free, Carrier Free, Bioactive, ActiBioPure™, Azide Free, His Tag, ≥95%(SDS-PAGE) ActiBioPure™,Animal Free,Azide Free,Bioactive,Carrier Free,His Tag 최소한의 기준선 간섭이 필요한 민감한 크로마토그래피 및 분석 워크플로우용.
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보관 및 배송
Store at -20°C,Avoid repeated freezing and thawing Ice chest + Ice pads 출고. 정확한 사양은 로트별 COA를 확인하세요.
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품질 문서
SDS, COA, 데이터시트, 사양서를 다운로드할 수 있습니다. 로트별 COA는 로트 번호 조회를 통해 확인할 수 있습니다.
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문헌적 근거
크로마토그래피, 유기 합성 및 교차 커플링 반응 분야의 동료 심사 논문 0편에서 인용되었습니다.
개요
Purity ≥95% (SDS-PAGE) Endotoxin level ＜0.1 EU/μg Function 5T4, also known as Trophoblast glycoprotein and Wnt-activated inhibitory factor 1 (WAIF1), is a single-pass transmembrane protein which may function as an inhibitor of Wnt/beta-catenin signaling (1). Distinct regions on the 5T4 ectodomain which contains eight leucine-rich repeats are thought to antagonize Wnt signaling (2). 5T4 has limited expression in normal adult tissues and in some solid tumors, while expression is elevated in human carcinomas which have been correlated with poor survival and prognosis (3-5). Human 5T4 is a 420 amino acid (aa) protein that includes a 31 aa signal peptide, a 324 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 44 aa cytoplasmic tail. Within the ECD, human 5T4 shares 78% and 77% aa sequence identity with mouse and rat 5T4, respectively. It has been identified that TIP-2/GIPC, a common interacting protein involved in cancer, interacts with 5T4 through its PDZ domain. 5T4 inhibits Wnt/ beta -catenin signaling by binding to LRP6 and inhibit Wnt-induced LRP6 internalization into endocytic vesicles. Conversely, 5T4 can activate noncanonical Wnt signaling through DKK1. In addition to LRP6, in-house data indicates 5T4 can also interact with Frizzled-8.
50 - 70 kDa, under reducing conditions; 50 -70 kDa, under non-reducing conditions.
분자 유형
Protein
Product Line
생물 제품
보관 및 배송
모양
Lyophilized
재구성
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
보관 조건
Store at -20°C,Avoid repeated freezing and thawing
배송
Ice chest + Ice pads
안정성 및 스토리지
Store at -20~-80℃ for more than 1 year. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
이미지
Recombinant Human 5T4 Protein (rp142398) - SDS-PAGE 3 μg/lane of Recombinant Human 5T4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 50 - 70 kDa.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
ZJ24F1215079
