RNAstore Sample Preservation Solution - BioReagent, ready-to-use, RNase free, sterile

Cat. No.: R665499

GRADE & PURITY BioReagent, Ready-to-use, RNase free, Sterile

Storage

Room temperature

Shipped In

Normal

Specifications

사양 및 순도

BioReagent, ready-to-use, RNase free, sterile

안정성 및 스토리지

Store at Room temperature for 24 months.

보관 조건

Room temperature

배송

Normal

등급

BioReagent, RNase free, 바로 사용 가능, 불임

Product Line

생물 제품

Application Protocols

No item-specific validated protocols are provided in the Product Data. The following high-level workflow outline is offered for context only and should be adapted to the official instructions supplied with the product and to your chosen RNA extraction kit.

General workflow (literature-based for RNA preservation solutions):

1. Prepare RNase-free tools and collection vessels. Wear fresh gloves.
2. Excise tissue or collect cells; immediately submerge or resuspend the sample in sufficient preservation solution to ensure full coverage.
3. Allow contact at room temperature for the recommended time (consult this product’s documentation). For thicker tissues, reduce piece size to aid diffusion.
4. Store the preserved sample per guidance (room temperature, refrigerated, or frozen), depending on timeline and sample size.
5. Prior to RNA extraction, remove excess solution. Proceed with the extraction protocol optimized for preserved samples.

For detailed ratios, times, and storage duration limits, refer to the product insert/CoA/Spec Sheet for this specific item (SKU R665499).

Biological Roles

The product itself is not a biomolecule with endogenous biological roles; it is a laboratory reagent designed to preserve RNA integrity ex vivo.

Functional context (general literature):

• RNA integrity in harvested samples is compromised by ubiquitous RNases and by autolysis processes. Preservation solutions help maintain transcript abundance patterns by limiting RNase activity and stabilizing nucleic acids during the pre-analytical phase.
• Stabilization is particularly important for labile mRNA species, small RNAs, and differential expression studies where pre-collection variables can bias results.
• Effective RNA preservation supports downstream applications such as qRT-PCR, RNA-seq, Northern blotting, and transcriptome profiling (research use), by reducing degradation-induced 3′/5′ bias and loss of low-abundance transcripts.

Mechanistic notes (general, not product-specific):

• Preservation strategies may include creating conditions that are unfavorable to RNase activity (e.g., ionic strength, water activity, or other stabilizing components) and helping maintain tissue architecture until extraction.

Item-specific performance claims (e.g., preservation duration at given temperatures, compatibility with particular extraction chemistries): Not specified for this item; refer to CoA/Spec Sheet.

Buffer Applications

This reagent is a preservation solution, not a general buffering agent for biochemical assays or electrophoresis.

• Typical buffer roles (pH control, ionic strength management for enzymes or chromatography) are not the intended use of this product.
• For running buffers (e.g., TAE/TBE), lysis buffers, or enzyme reaction buffers, choose dedicated formulations designed for those purposes.

Workflow note: After preservation, samples are typically transferred to appropriate lysis/binding buffers provided by RNA extraction kits. Consult the extraction protocol for any steps requiring washing to remove residual preservation solution before enzymatic reactions.

Green Alternatives

Sustainability considerations for RNA stabilization workflows focus on cold-chain minimization and reagent safety.

• Room-temperature logistics: This product is specified for room temperature storage, which can reduce reliance on dry ice or ultracold transport during the preservation step, lowering energy and CO₂ footprint relative to snap-freezing-only workflows (operational sustainability).
• Aqueous matrix: RNA preservation solutions are typically water-based (general literature), which may reduce the volume of flammable organic solvents in the lab. However, always consult the SDS for any hazardous components and disposal requirements.
• Waste minimization: Using a ready-to-use sterile/RNase-free formulation can decrease failed extractions and repeat sampling, indirectly reducing consumables and waste.

Trade-offs (general):

• Some preservation reagents can contain salts or other additives that require additional wash steps during extraction, potentially increasing plastic consumables.
• Cold storage of extracted RNA is still necessary; preservation solutions focus on the pre-extraction interval.

If your institution has green lab initiatives, consider:

• Consolidated shipments (this item ships under normal conditions).
• Proper segregation of aqueous chemical waste per local rules to enable optimal treatment.

Item-specific environmental, health, and safety profile: Not specified for this item; refer to SDS.

Pharmaceutical Uses

This product is sold for research use only and is not intended for human or veterinary use, diagnostic procedures, or therapeutic applications.

• It is not designated as a pharmaceutical excipient, and no pharmacopeial monograph applicability is claimed.
• Use is limited to laboratory workflows involving collection and preservation of research specimens for subsequent RNA isolation and analysis.

Any cGMP, clinical validation, or regulatory status for clinical sample handling: Not specified for this item; refer to CoA/Spec Sheet if applicable.

Physical Properties

Item-specific physicochemical specifications are not provided in the Product Data.

Item-specific values:

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• pH, osmolality/tonicity, and ionic composition: Not specified for this item; refer to CoA/Spec Sheet.
• Density, refractive index, conductivity: Not specified for this item; refer to CoA/Spec Sheet.

General/literature expectations for RNA preservation solutions (for context, not item specifications):

• Matrix: Aqueous solution (water-based)
• Physical state: Clear liquid (typical for such reagents)
• Approximate density: ~1.0 g/mL at 20–25 °C (literature for dilute aqueous solutions)
• Boiling/Freezing behavior: Near that of water; additives can shift freezing point/boiling range (literature)
• Solubility: Miscible with water (typical); compatibility with alcohols varies by formulation (literature)

Use relevance:

• Room-temperature stability of RNA in treated samples is a key functional attribute of this product class; however, exact stability windows and temperature limits for this item are not specified here and should be confirmed from the manufacturer documentation.

Always defer to the CoA/Spec Sheet for parameter ranges that control sample integrity (e.g., allowable storage temperature/time for preserved tissues, cells, or RNA).

Quality and Grades

• Grade: BioReagent — suitable for routine laboratory workflows in molecular biology and biochemistry. Emphasis is on performance in biological sample handling rather than analytical trace-metal or chromatographic specifications.
• Format: Ready-to-use — no dilutions or additives are required prior to use under standard workflows (minimizes handling variability and RNase exposure).
• Critical attributes: RNase free, Sterile — indicates the product is manufactured and packaged to minimize contaminating ribonuclease activity and microbial burden, which is essential for preserving RNA integrity.

What this means in practice:

• RNase-free: Production and QC are designed to prevent introduction of RNases. Users must also maintain RNase-free technique during handling (fresh gloves, dedicated tools, RNase-free tubes and tips) to preserve performance.
• Sterile: Suitable for contact with biological tissues and cell pellets prior to RNA extraction, helping to minimize degradation introduced by microbial enzymes.
• Ready-to-use: Eliminates lot-to-lot variability that can arise from lab-prepared formulations and reduces exposure time of samples to the environment.

Item-specific specifications (acceptance criteria, RNase activity limits, sterility test method, bioburden limits, UV absorbance background): Not specified for this item; refer to CoA/Spec Sheet.

Note: This product is intended for research use only and is not certified for clinical diagnostic or therapeutic applications.

Reaction and Applications

This product is used in biological sample handling rather than in chemical synthesis. Its primary application is preserving RNA integrity in tissues, cells, or biofluids between collection and extraction.

Key application contexts (general literature for RNA preservation reagents):

• Field or bench-top stabilization of samples to limit RNase-mediated degradation prior to processing.
• Staging of sample logistics where immediate freezing is impractical; enables subsequent extraction using standard kits.
• Facilitates consistent transcript profiles by minimizing pre-analytical variability.

Practical tips (general, not item-specific):

• Use RNase-free tools and containers. Change gloves frequently to prevent RNase transfer.
• Ensure sufficient reagent-to-sample ratio so the solution adequately penetrates tissue/cell pellets. Thick tissues are typically trimmed to smaller pieces to aid diffusion (literature best practice).
• Allow adequate contact time at room temperature for stabilization before any cold storage transfer, as recommended by your workflow or extraction kit.
• Prior to extraction, remove or decant excess preservation solution as directed by your chosen protocol to avoid interfering with lysis/binding steps.

Manufacturer Applications: Not provided beyond the product name. For exact use instructions, incubation times, and compatibility notes for this item, refer to the product insert, CoA/Spec Sheet, or SDS.

Reaction Conditions

Instead of chemical reaction conditions, the relevant operational parameters concern sample preservation conditions. Item-specific instructions are not provided in the Product Data; consult the product insert for authoritative parameters. The following are general literature practices for RNA preservation solutions and are not item specifications:

• Sample-to-reagent ratio: Ensure an excess volume of preservation solution relative to tissue mass or cell pellet size to promote diffusion and complete coverage. Trim tissues to small pieces to improve penetration.
• Temperature: Many preservation workflows allow room-temperature contact during the initial stabilization interval; longer-term storage may involve refrigeration or freezing of the preserved sample per protocol. Confirm limits for this item in its documentation.
• Contact time: Allow sufficient equilibration time for the solution to permeate the sample before downstream freezing or processing (time depends on sample size and formulation; see product-specific guidance).
• Downstream processing: Prior to lysis, remove or decant residual preservation solution as required by your RNA extraction kit to prevent interference with binding/wash chemistries.

Compatibility notes (general): Preserved samples are commonly compatible with silica-membrane or magnetic-bead RNA kits and standard phenol/chaotrope protocols; always check the kit manual for any special steps when starting from preserved material.

Safety and Handling

Hazard classification for this specific item is not provided in the Product Data. Handle using good laboratory practices and consult the SDS for authoritative guidance.

GHS information (item-specific):

• Signal word: Not specified for this item; refer to SDS.
• Hazard statements (H-codes): Not specified for this item; refer to SDS.
• Pictograms / classification: Not specified for this item; refer to SDS.

General laboratory handling guidance (applicable to aqueous molecular biology reagents):

• Wear appropriate PPE: lab coat, safety glasses, disposable nitrile gloves. Use RNase-free technique to avoid contaminating the reagent and your samples.
• Work area: Use a clean, RNase-free workspace; wipe down benches and tools with RNase-decontamination solutions when appropriate.
• Incompatibilities: Do not mix unknown preservation formulations with oxidizers or hypochlorite bleach; reactive byproducts can form with certain organic/amine/thiocyanate-containing solutions. If decontamination with bleach is needed, thoroughly rinse away any reagent residues first. Always confirm compatibility in the SDS.
• First aid (overview; consult SDS): If on skin or in eyes, rinse with water. If inhaled as aerosol/mist, move to fresh air. If ingested, seek medical advice and show SDS.
• Spill response: Absorb with inert material, collect for disposal, then wash area with water while observing RNase-contamination control.
• Waste: Dispose according to institutional and local regulations for aqueous laboratory chemical waste.

Storage: See the Storage & Reconstitution tab for item-specific instructions.

Solvent Selection

This is a specialized, ready-to-use aqueous preservation reagent rather than a general-purpose organic solvent. Solvent selection in synthetic chemistry is therefore not typically applicable.

For end users, relevant considerations are:

• Matrix: Water-based (literature for this reagent class). Compatible with typical RNA extraction workflows following preservation.
• Downstream compatibility: Generally used prior to lysis with chaotropic agents or phenol/guanidinium-based methods, or column-based kits (general literature). Always follow the extraction protocol guidance for handling preserved samples.

If you require a solvent for chemical reactions, consult other product listings (e.g., alcohols, aprotic solvents). For sample preservation tasks, refer instead to the Reaction & Applications and Application Protocols sections for workflow-relevant guidance.

Storage and Reconstitution

• Storage temperature (item-specific): Room temperature (per Product Data).
• Shipping: Normal conditions (per Product Data).
• Format: Ready-to-use; no reconstitution required.

Best practices:

• Maintain the container tightly closed to prevent evaporation and contamination. Avoid introducing RNases by using RNase-free pipette tips and changing gloves before handling.
• If aliquoting for frequent use, pre-rinse RNase-free tubes with the solution to minimize adsorption losses (general practice) and label aliquots with open dates.
• Avoid repeated opening in dusty or high-traffic areas; consider handling in a clean area or biosafety cabinet if working with primary tissues.
• Do not freeze unless the product documentation explicitly permits it; freeze–thaw can affect performance in some formulations.

Stability and shelf life for this specific item, as well as acceptable temperature excursions and light sensitivity: Not specified for this item; refer to CoA/Spec Sheet.

Research Use Only: This product is intended for research use only.

Structure and Identity

This product is a formulated, ready-to-use aqueous reagent designed for stabilization of RNA in biological samples. It is a mixture rather than a single defined compound, and therefore does not have a single molecular structure.

• Product type: Proprietary aqueous preservation solution (RNase-free, sterile)
• Composition details: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula: Not applicable to mixtures (no single formula)
• Molecular weight: Not applicable to mixtures (no single MW)
• SMILES / InChI / InChIKey: Not applicable to mixtures

2D structural features (descriptive): As a solution, the identity is defined by its functional performance (RNA preservation and RNase inactivation control) rather than discrete functional groups of a single molecule. The formulation is typically water-based and may include salts or stabilizing agents (general literature), but the exact constituents for this product are not disclosed.

Naming and catalog identity:

• SKU: R665499
• Product Name: RNAstore Sample Preservation Solution
• Grade: BioReagent, Ready-to-use, RNase free, Sterile
• Category: Life Science (研究/实验用)

Note: For structural/compositional specifics of this exact item, consult the product’s CoA/Specification Sheet or SDS.

Synthetic Utility

Not applicable. This is a biological sample preservation solution and is not used as a reagent in chemical synthesis or in named organic reactions.

For synthetic chemistry applications (e.g., as solvent, nucleophile, base/acid, or coupling reagent), please consult appropriate chemical reagents and solvents in the catalog. For this product, the relevant expertise areas are molecular biology sample handling and nucleic acid extraction workflows rather than organic synthesis.

Target Specificity

Not applicable. This product is not an antibody or affinity reagent and has no antigen/epitope specificity.

Item-specific details (antigen, clone, isotype, species reactivity): Not specified for this item.

자주 묻는 질문

How is this product shipped?

This product ships under standard ambient conditions. No temperature-controlled packaging is required.