Sabouraud Dextrose Agar Medium (USP) - BioReagent

Cat. No.: S1528274

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

개요

For mold and yeast enumeration and cultivation. Preparation method: Weigh 65.0 g of this product in 1 L of purified water, stir and heat to boiling until completely dissolved, autoclave at 121°C for 15 minutes, and set aside for use.

Specifications

사양 및 순도

BioReagent

보관 조건

Room temperature

배송

Normal

등급

BioReagent

Product Line

biological_products

Application Protocols

The following are illustrative microbiology protocols (literature) for Sabouraud Dextrose Agar; adapt to your SOPs. Item-specific instructions are Not specified; refer to CoA/Spec Sheet.

• A. Preparation of SDA plates

  1. Weigh dehydrated medium to yield ~65 g/L in purified water; stir to disperse.
  2. Heat with frequent swirling to dissolve completely; avoid scorching.
  3. Autoclave at 121 °C for 15 minutes.
  4. Cool to 50–55 °C; optionally add sterile-filtered supplements (e.g., chloramphenicol) as required by your method.
  5. Pour 20–25 mL per 90-mm plate. Allow to solidify and dry before use.

• B. Environmental monitoring/contact plates (general)

  • Prepare plates with slightly overfilled volumes to create convex surfaces; dry to reduce excessive moisture. Use according to site EM program.

• C. Slants for stock maintenance

  1. Dispense 8–10 mL into screw-cap tubes; sterilize as above.
  2. Solidify on a slant; inoculate and incubate per organism requirements.

• D. Broth version

  • Omit agar to prepare Sabouraud Dextrose Broth for enrichment; sterilize and supplement as required.

• E. Storage of prepared media

  • Store plates inverted in sealed sleeves at 2–8 °C; allow to equilibrate to room temperature before use. Shelf-life is method- and lab-specific; Not specified for this item—verify with internal QC.

Biological Roles

Sabouraud Dextrose Agar (SDA) is a nutritive, acidic medium formulated to favor fungi. It does not itself have a biological role in vivo; rather, its components support in vitro growth.

• Functional contributions (literature)

  • Dextrose (glucose): primary carbon and energy source promoting rapid yeast growth and supporting mold development.
  • Peptones (casein/animal/vegetable digests): supply amino acids, peptides, and growth factors required for biomass accumulation and enzyme synthesis.
  • Agar: inert gelling agent from red algae; provides a stable surface for colony isolation, with minimal microbial metabolism.

• Selectivity mechanism

  • The typical final pH of ~5.6 suppresses many bacterial species while permitting growth of acid-tolerant yeasts and filamentous fungi. When necessary, supplementation with antibacterial agents enhances selectivity.

• Use in laboratory ecology (general)

  • Supports recovery of environmental and product-associated fungal communities for enumeration, identification, and strain maintenance.

• Relationship to physiology

  • Carbohydrate-rich, low-pH conditions can influence fungal morphology (e.g., budding patterns in yeasts, sporulation tendencies in molds) and pigment production, which are often used as phenotypic cues during identification. Exact behaviors vary widely by organism and are determined empirically per strain and incubation parameters.

Buffer Applications

This product is not a buffering reagent and is not typically used to prepare defined pH buffer systems for biochemical assays.

• Practical note

  • SDA exhibits an acidic working pH (literature: ~5.6 after sterilization) due to its formulation, but it does not have a defined buffer capacity like citrate, acetate, or phosphate systems. If a buffered fungal medium is required, consider defined media supplemented with appropriate buffer salts.

• Recommendation

  • For pH-critical experiments, prepare separate standardized buffers or use buffered media formulations specified by your method. SDA should be used primarily for cultivation, isolation, and enumeration of yeasts and molds.

Green Alternatives

Although culture media are not solvents or classical reagents with direct green-replacement frameworks, sustainability considerations apply to preparation and disposal.

• Resource and waste minimization (general)

  • Dehydrated format reduces transport mass and packaging compared to pre-poured plates; prepare only what you need to minimize expired-plate waste.
  • Use energy-efficient autoclave cycles validated for load size; avoid unnecessary extended sterilizations that increase energy use and can degrade media.
  • Opt for reusable glassware (flasks, bottles, plate-casting manifolds) when compatible with your QA requirements to reduce single-use plastics.

• Media choice considerations (literature)

  • For mold/yeast enumeration, SDA, Malt Extract Agar (MEA), and Potato Dextrose Agar (PDA) are commonly interchangeable depending on the matrix and organism. Selection based on method suitability can reduce repeat testing and associated waste.

• End-of-life

  • Decontaminate spent media via autoclaving before disposal. Solidify and dewater agar-containing waste where permitted to reduce landfill mass. Follow local regulations for biohazardous waste.

• Chemical footprint

  • Avoid unnecessary antibiotic supplements unless method-required; this reduces risks of resistance selection and downstream ecotoxicity. When antibiotics are necessary, prepare small batches and store properly to minimize expired waste.

Pharmaceutical Uses

No therapeutic or clinical use. For research and laboratory QC only.

• Role in pharmaceutical microbiology (literature)

  • Commonly used for microbial enumeration testing (yeasts and molds) of nonsterile products, raw materials, and environmental monitoring plates in alignment with compendial microbiology (e.g., USP general chapters such as <61> and <62> when method suitability is demonstrated). Specific compliance of this item is Not specified; refer to CoA/Spec Sheet and your QA procedures.
  • Employed in growth promotion testing (GPT) as part of media qualification using reference strains (e.g., Candida albicans) to verify fertility and selectivity.

• Formulation/processing context

  • Used to assess bioburden and product cleanliness during development and manufacturing; not part of finished drug products.

• Documentation

  • Maintain traceability records: supplier, catalog number (SKU S1528274), lot, prep date, sterilization parameters, and GPT results. Archive CoAs for audits.

• Limitations

  • Not a defined medium; composition variability between peptone sources can influence recovery of some organisms. Method suitability should justify its use for the specific product matrix under test.

Physical Properties

Because this is a dehydrated culture medium (mixture), single-compound constants (bp, mp, logP) are not applicable.

• Item-specific properties

  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • Density, moisture content, particle size: Not specified for this item; refer to CoA/Spec Sheet.

• Preparation-related properties (literature, typical for USP Sabouraud Dextrose Agar)

  • Working concentration (dehydrated → ready-to-use agar): commonly about 65 g per liter of purified water to yield a firm gel; actual preparation for this item Not specified; verify CoA/Spec Sheet.
  • Final pH (25 °C): typically 5.6 ± 0.2 after sterilization (literature, USP description). The acidic pH suppresses many bacteria while supporting fungi.
  • Solubility/dispersion: Disperses in cold water; requires heating to near-boil with agitation for complete dissolution; agar solidifies on cooling (~32–40 °C) and melts at ~85–95 °C (literature behavior of agar gels).
  • Appearance of poured medium: clear to slightly opalescent, light amber (literature), depending on water quality and peptone source.

• Stability (general)

  • Dehydrated powder is hygroscopic; exposure to humidity can cause caking and performance drift. Store tightly sealed.
  • Prepared plates/slants are stable for limited periods when protected from dehydration and stored refrigerated; exact shelf-life Not specified for this item; consult internal QC guidance.

Quality and Grades

• Grade (from Product Data)

  • BioReagent: suitable for laboratory research applications where consistent performance and low adventitious contaminants are required for microbiological work. BioReagent-grade media emphasize lot-to-lot reproducibility and functional performance in cultivation assays.

• USP designation in name (context, not a claim)

  • “Sabouraud Dextrose Agar (USP)” reflects the commonly referenced medium used in compendial microbiology (e.g., USP chapters for microbial enumeration of nonsterile products). For this specific item, explicit conformity tests and specifications are Not specified; refer to CoA/Spec Sheet.

• What quality means for SDA (general expectations, literature)

  • Performance: ability to support luxuriant growth of representative fungi (e.g., Candida spp., Aspergillus spp.) while suppressing many bacteria due to acidity; recovery of stressed/low bioburden organisms in pharmaceutical and cosmetic matrices when appropriately supplemented.
  • Clarity/gel strength: agar quality impacts colony morphology and streaking characteristics; consistent gelation is vital for enumeration.
  • Background contaminants: low bioburden and low inhibitory residues (e.g., heavy metals, peroxide byproducts) are important; specific limits for this item are Not specified; refer to CoA/Spec Sheet.

• Documentation and controls

  • Request lot-specific CoA for pH after sterilization, growth-promotion results, gel strength, and appearance. Retain records of media preps (date, lot, sterilization cycle) for traceability in regulated workflows.

Reaction and Applications

This material is not used as a reagent for chemical synthesis; instead, its applications are microbiological.

• Primary uses (literature)

  • Cultivation, isolation, and enumeration of yeasts and molds from clinical, environmental, food, cosmetic, and pharmaceutical samples.
  • Growth Promotion Testing (GPT) and method suitability studies aligned with compendial microbiology (e.g., USP general chapters) when appropriate controls are used.
  • Stress recovery: acidic pH (~5.6) and high carbohydrate content support recovery of fungi that may be stressed by product matrices.

• Selective/diagnostic adaptations (general)

  • Antibiotic-supplemented SDA to inhibit bacteria (e.g., chloramphenicol) or dermatophyte-focused variants (e.g., with cycloheximide, per method requirements). Supplement choices must follow institutional/compendial guidance.
  • Employed as slants for maintenance of fungal stocks; as plates for colony counts; or converted to broth by omitting agar for enrichment.

• Practical tips

  • Ensure homogenous dissolution and complete sterilization (e.g., 121 °C, 15 min; literature) to avoid batch-to-batch performance variability. Do not overheat; caramelization may darken medium and reduce performance.
  • Validate water quality and autoclave performance; record lot numbers and sterilization cycles for traceability.
  • For enumeration, dry plates surface before inoculation to prevent colony spreading; incubate at method-specified temperatures (often 20–25 °C or 25–30 °C for molds/yeasts; literature). Exact conditions for this item are not specified; follow your method SOP.

Reaction Conditions

This section is adapted to preparation and use conditions for microbiological media rather than chemical reactions.

• Reconstitution and sterilization (literature example; not item-specific)

  • Typical working concentration: approximately 65 g/L in purified water. Exact directions for this item are Not specified; refer to CoA/Spec Sheet.
  • Dissolve with heat while stirring; boil gently until completely dissolved.
  • Distribute to flasks or bottles; autoclave at 121 °C for 15 minutes (15 psi). Avoid prolonged sterilization to prevent sugar caramelization.

• Plate pouring (general)

  • Cool autoclaved medium to ~50–55 °C. If using antibiotic supplements, aseptically add sterile-filtered solutions at this stage.
  • Pour into sterile petri dishes (typically 20–25 mL per 90-mm plate). Allow to solidify; dry surface (e.g., 30–60 minutes in a laminar-flow hood) before use or storage.

• Incubation (application-dependent; literature)

  • For yeasts: often 25–30 °C for 24–48 h.
  • For molds: often 20–25 °C for 3–7 days, or per compendial method. Exact conditions depend on organism and method SOP.

• Quality checks

  • Perform Growth Promotion Testing with appropriate strains (e.g., Candida albicans) and negative controls. Inspect for color, clarity, gel strength, and sterility before use.

Safety and Handling

• GHS/Classification (item-specific)

  • Signal Word: Not specified for this item; refer to SDS.
  • Hazard Statements / Pictograms / GHS Classification: Not specified for this item; refer to SDS.

• General laboratory safety (applicable to dehydrated microbiological media)

  • Avoid dust generation and inhalation; wear appropriate PPE: lab coat, safety glasses, and disposable nitrile gloves. Use local exhaust ventilation when weighing to minimize aerosolization.
  • Hygiene: Wash hands after handling. Do not eat, drink, or smoke in work areas.
  • Incompatibilities: Strong oxidizers and strong acids/bases can degrade organic constituents. Keep away from moisture to prevent caking and microbial contamination.

• Thermal handling

  • During preparation, hot media and glassware can cause burns. Use heat-resistant gloves and eye/face protection when boiling and pouring plates. Beware of steam during autoclaving and depressurization.

• Microbiological risk

  • The dehydrated powder is generally nonpathogenic; however, once inoculated, cultures may contain opportunistic pathogens or allergenic fungal spores. Handle inoculated materials under appropriate biosafety level practices (commonly BSL-2 for unknown environmental isolates). Disinfect work surfaces and properly autoclave all biohazardous waste.

• First-aid overview (general guidance; defer to SDS)

  • Inhalation of dust: move to fresh air, seek medical attention if symptoms persist.
  • Skin/eye contact: rinse with water for several minutes; remove contaminated clothing. Seek medical advice if irritation develops.
  • Ingestion: rinse mouth; do not induce vomiting; obtain medical attention if unwell.

Solvent Selection

This product is a dehydrated culture medium; solvent selection pertains to its reconstitution rather than chemical reaction workups.

• Primary solvent (applicable)

  • Purified water is the standard solvent for reconstitution. Use water meeting your lab’s microbiology grade (e.g., sterile purified water, ASTM Type I/II) to minimize inhibitory ions or organics.

• Practical guidance (general)

  • Dissolution: sprinkle powder into vigorously stirred cold water to avoid clumping; bring to a near boil for complete dissolution.
  • Gelation: agar solidifies upon cooling; pour plates at ~50–55 °C to reduce condensation and preserve supplements.
  • Additives: For selective applications, sterile-filtered antibiotics (e.g., chloramphenicol, gentamicin) are added after autoclaving and cooling to ~50 °C; choice depends on the target organisms and regulatory method. Selection details are application-specific and not specified for this item.

• Comparison (literature)

  • SDA vs. Potato Dextrose Agar (PDA): SDA’s lower pH and peptone content tend to favor clinical yeasts/molds; PDA (potato infusion) is often preferred for sporulation and plant pathogens.
  • SDA vs. Malt Extract Agar (MEA): MEA provides rich carbohydrates from malt; SDA’s defined dextrose and peptone base can offer more consistent colony morphology for some QC assays.

Storage and Reconstitution

• Item-specific storage and shipping (from Product Data)

  • Storage Conditions: Room temperature.
  • Shipped In: Normal.

• Handling and shelf-life (general)

  • Keep dehydrated powder tightly closed in a cool, dry place. Protect from humidity and microbial contamination. Avoid frequent opening cycles; use in a desiccated weighing station when possible. Item-specific shelf-life Not specified; refer to CoA/Spec Sheet.

• Reconstitution (literature example; not item-specific)

  • Weigh approximately 65 g of dehydrated medium per liter of purified water. Disperse with stirring, heat to dissolve, and sterilize by autoclaving at 121 °C for 15 min. Cool to 50–55 °C before pouring plates or adding heat-labile supplements.
  • For broth, omit agar and keep the same nutrient concentration; sterilize as above.

• Prepared media storage (general)

  • Plates: store inverted at 2–8 °C in sealed sleeves to prevent desiccation and contamination. Allow condensation to dissipate before inoculation.
  • Slants/broth: store at 2–8 °C. Avoid freezing agar-based media.
  • Usable period depends on in-house QC (appearance, pH, sterility, growth-promotion). Specific recommendations for this item are Not specified; consult your lab’s validation data.

• Research Use Note

  • For research use only.

Structure and Identity

Sabouraud Dextrose Agar (SDA) is a complex, dehydrated culture medium blend rather than a single defined chemical entity.

• Item identity (from Product Data)

  • Product: Sabouraud Dextrose Agar Medium (USP)
  • Grade: BioReagent
  • CAS: Not applicable (mixture)
  • SMILES / InChI / InChIKey: Not applicable (mixture)
  • Molecular formula / Molecular weight: Not applicable to a mixture; Not specified for this item; refer to CoA/Spec Sheet.

• Structural features (general description, literature)

  • Composition is typically a mixture of protein hydrolysates (peptones), dextrose (glucose) as a fermentable carbohydrate, and agar as a gelling polysaccharide matrix.
  • As a complex medium, individual components contain heterogeneous macromolecules (peptides, amino acids, carbohydrates) and inorganic salts without a single definable molecular structure.

• Formulation context (literature)

  • “Sabouraud” media are formulated to favor yeasts and molds via high dextrose and an acidic final pH. Agar provides a semi-solid surface for colony development; peptones supply nitrogen, vitamins, and growth factors.
  • The suffix “(USP)” denotes alignment with the general characteristics of the United States Pharmacopeia medium used for microbiological quality testing; specific conformity for this item is Not specified; refer to CoA/Spec Sheet.

• 2D description in words (general)

  • Blend of fine, free-flowing tan-to-beige dehydrated powder containing: soluble nutrients (peptones, dextrose) and insoluble/colloidal gelling polymer (agar) that hydrates and forms a gel upon boiling and cooling.

Synthetic Utility

Not applicable for chemical synthesis.

• Context

  • Sabouraud Dextrose Agar is a complex biological medium intended for cultivation of fungi, not a functional reagent for organic or inorganic transformations. It does not participate as a reagent, ligand, catalyst, or building block in chemical reactions.

• Guidance

  • For chemical or biochemical synthesis involving carbohydrates or peptides, use defined reagents (e.g., D-glucose, amino acids, protected peptides, agarose) rather than complex media. For microbial biotransformations, SDA may be used to grow source organisms, but reaction execution and analysis occur in separate, defined systems.

Target Specificity

This section is primarily relevant to antibodies or affinity reagents and is not applicable to culture media.

• For SDA, “specificity” refers broadly to growth preference rather than molecular target binding. By design (literature), its acidic pH and nutrient profile favor the growth of yeasts and filamentous fungi while suppressing many bacteria. No antigen/epitope/clone/isotype information applies.