등급 & 순도Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.1.0 mg/mL
Carrier Free, ExactAb™, Validated, 1.0 mg/mL Carrier Free,ExactAb™,Validated 최소한의 기준선 간섭이 필요한 민감한 크로마토그래피 및 분석 워크플로우용.
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보관 및 배송
Store at -80°C,Avoid repeated freezing and thawing Dry ice packs + Cold packs 출고. 정확한 사양은 로트별 COA를 확인하세요.
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품질 문서
SDS, COA, 데이터시트, 사양서를 다운로드할 수 있습니다. 로트별 COA는 로트 번호 조회를 통해 확인할 수 있습니다.
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문헌적 근거
크로마토그래피, 유기 합성 및 교차 커플링 반응 분야의 동료 심사 논문 0편에서 인용되었습니다.
개요
Mouse anti Human SCAMP5 Antibody, Monoclonal (K06322_12G11), could be used for WB, IHC, IP and so on. Application: WB: 1/1000 IHC: 1/200 IP: 1/100 Protein Function Required for the calcium-dependent exocytosis of signal sequence-containing cytokines such as CCL5. Probably acts in cooperation with the SNARE machinery. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT) in case of endoplasmic reticulum stress by inhibiting the endocytosis pathway.
사양
Product Name
SCAMP5 Mouse mAb - 1차 항체, specific to SCAMP5, Mouse IgG1
Store at -80°C,Avoid repeated freezing and thawing
배송
Dry ice packs + Cold packs
안정성 및 스토리지
Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
이미지
SCAMP5 Mouse mAb (Ab126689) - Western Blot All lanes: SCAMP5 Mouse mAb (Ab126689) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution
Predicted band size: 26 kDa Observed band size: 24 kDa Exposure time: 100.0 s
SCAMP5 Mouse mAb (Ab126689) - IHC IHC analysis of cerebellum tissue by SCAMP5 Mouse mAb (Ab126689). IHC was performed using sections of the formalin-fixed paraffin-embedded cerebellum tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with SCAMP5 Mouse mAb (Ab126689) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Cytoplasm of cells in granular layer and in molecular layer are positively stained.
SCAMP5 Mouse mAb (Ab126689) - IP Immunoprecipitation was performed by incubation of 2.5 µg of SCAMP5 Mouse mAb (Ab126689) with mouse brain lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. SCAMP5 Mouse mAb (Ab126689) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. Lane 1: Mouse brain lysate Lane 2: SCAMP5 immunoprecipitated from mouse brain lysate by SCAMP5 Mouse mAb (Ab126689) Lane 3: The same as Lane 2 but Mouse IgG was used as IgG isotype control antibody Result: SCAMP5 Mouse mAb (Ab126689) can immunoprecipitate SCAMP5.
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