Determine the necessary mass, volume, or concentration for preparing a solution.
최소한의 기준선 간섭이 필요한 민감한 크로마토그래피 및 분석 워크플로우용.
Store at -20°C,Avoid repeated freezing and thawing Ice chest + Ice pads 출고. 정확한 사양은 로트별 COA를 확인하세요.
SDS, COA, 데이터시트, 사양서를 다운로드할 수 있습니다. 로트별 COA는 로트 번호 조회를 통해 확인할 수 있습니다.
크로마토그래피, 유기 합성 및 교차 커플링 반응 분야의 동료 심사 논문 0편에서 인용되었습니다.
Product content
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Product Introduction
Super DNA Marker consists of 10 DNA fragments, 10,000bp, 5,000bp, 3,000bp, 2,000bp, 1,500bp, 1,000bp, 750bp, 500bp, 250bp, and 100bp respectively, and it can be directly electrophoresed with 5μl of 1×Loading Buffer. This product is a DNA solution containing 1×Loading Buffer, 5μl of which can be taken for direct electrophoresis, which is very convenient to use; the amount of DNA fragment of 1,000bp in electrophoresis is about 150ng, which shows a bright band, and the amount of DNA in other bands is about 50ng.
Pre-experiment Preparation and Important Notes
1. This product can be directly frozen and mixed for use without heating.
2. Please change the electrophoresis buffer and use fresh agarose gel in time to avoid affecting the electrophoresis results.
3. When DNA agarose gel electrophoresis, the purity of the gel has a great influence on the clarity of the DNA bands, so try to use good quality agarose, the recommended gel concentration is 1%-3%, voltage 4-8v/cm.
4. The gel concentration is closely related to the separation performance of DNA fragments. The larger the gel concentration, the better the separation performance of short fragment DNA; conversely, the smaller the gel concentration, the more favorable the separation of long fragment DNA.
5. When used with macromolecular dyes, it is recommended to appropriately reduce the amount of Marker or increase the amount of dye.
Usage
Take 5 μl into the spiking well of the agarose gel (if the spiking well is wider than 6 mm, the amount of sample must be increased appropriately), and carry out electrophoresis.
experimental result

1% agarose gel electropherogram (5 μ L)
위험성, 취급, 보관 및 규제 준수를 포괄하는 종합 문서.
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