Syntaxin 18 Mouse mAb - 1차 항체, specific to STX18

Cat. No.: Ab130021

등급 & 순도 ExactAb™, See COA

Application

IHC, WB

Host species

마우스

Reactive species

마우스, 인간, 조언

Isotype

Mouse IgG1

Clone Number

OTI3D7

Conjugation

Unconjugated

개요

Mouse anti Human Syntaxin 18 Antibody, Monoclonal (OTI2B5), could be used for WB and so on.

사양

Product Name

Syntaxin 18 Mouse mAb - 1차 항체, specific to STX18

동의어

Ufe1|Cell growth inhibiting gene 9 protein antibody|Cell growth-inhibiting gene 9 protein antibody|DKFZp686O15149 antibody|STX18 antibody|Syntaxin-18 antibody|Ufe1 antibody

사양 및 순도

ExactAb™,See COA

생화학 및 생리적 메커니즘

Syntaxin that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER.

숙주 종

마우스

특이성

STX18

면역원

Recombinant protein of human STX18 produced in E.coli (AA 1-285).

포지티브 제어

WB: HeLa, MCF7, K562 and Hep G2 cell lysates. IHC: Human endometrium tissue, human endometrial adenocarcinoma tissue, and human lymph node tissue.

변화

Unconjugated

등급

ExactAb™

SDS-PAGE

150 kDa

정화 방법

Protein G purified

집중력

See COA

Product Line

생물 제품

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: Syntaxin 18 Mouse mAb (Ab130021)

Syntaxin 18 Mouse mAb (Ab130021) - Western Blot 

All lanes: Syntaxin 18 Mouse mAb (Ab130021) at 1/1000 dilution 

Samples: Lysates at 20 µg per lane 

Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution 

Predicted band size: 39 kDa 

Observed band size: 42 kDa 

Exposure time: 3.4 s

Western Blot: Syntaxin 18 Mouse mAb (Ab130021)

Syntaxin 18 Mouse mAb (Ab130021) - Western Blot 

All lanes: Syntaxin 18 Mouse mAb (Ab130021) at 1/1000 dilution 

Samples: Lysates at 20 µg per lane 

Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution 

Predicted band size: 39 kDa 

Observed band size: 42 kDa 

Exposure time: 3.5 s

IHC: Syntaxin 18 Mouse mAb (Ab130021)

Syntaxin 18 Mouse mAb (Ab130021) - IHC

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using Syntaxin 18 Mouse mAb (Ab130021). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

IHC: Syntaxin 18 Mouse mAb (Ab130021)

Syntaxin 18 Mouse mAb (Ab130021) - IHC

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using Syntaxin 18 Mouse mAb (Ab130021). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

IHC: Syntaxin 18 Mouse mAb (Ab130021)

Syntaxin 18 Mouse mAb (Ab130021) - IHC

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using Syntaxin 18 Mouse mAb (Ab130021). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

적용

적용

Dilution info

WB

1/500-1/2000

IHC

1/2000

관련 대상(인간)

STX18 Tbio Syntaxin-18 (0 Activities)

Discover Target: STX18

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

자주 묻는 질문

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Syntaxin 18?Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.