Tanshindiol C - ≥98% , CAS No.97465-71-9

CAS: 97465-71-9 Cat. No.: T648257 분자식: C₁₈H₁₆O₅ 분자량: 312.32 PubChem CID: 126072

GRADE & PURITY ≥98%

Storage

Protected from light,Store at -80°C

Shipped In

Dry ice packs + Cold packs

개요

Tanshindiol C is a S-adenosylmethionine-competitive EZH2 ( Histone Methyltransferase ) inhibitor with an IC 50 of 0.55 μM for inhibiting the methyltransferase activity. Tanshindiol C is also an activator of both Nrf2 and Sirtuin 1 (Sirt1) in macrophages. Tanshindiol C possesses anti-cancer activity, and can be used for atherosclerosis research

In Vitro

Tanshindiol C (1-10 μM; for 24 h) activates Nrf2 and upregulates Prdx1 expression and mRNA levels in macrophages. Tanshindiol C protects macrophages from oxidized low-density lipoprotein (oxLDL) induced foam cell formation via activation of Prdx1/ABCA1 signaling. Tanshindiol C inhibits both wild-type and A667G mutant (K i value of 176 nM) EZH2 activity with similar potencies. Tanshindiol C inhibits growth of the cell lines such as Pfeiffer, U-2932 and Daudi (lymphoma), PC3 (prostate cancer), T98G and U87MG (glioma), and A549 (lung cancer), with GI 50 values of 1.5 μM, 9.5 μM, 10.6 μM, 4 μM, 6 μM, 5.7 μM and 4.2 μM, respectively. Tanshindiol C (1-5 μM; 72 hours) induces accumulation of Pfeiffer cells in sub-G1 phase, which indicates cells in late apoptosis and necrosis. Tanshindiol C (1-3 μM; 72 hours) increases protein levels of the important apoptosis related protein markers, cleaved caspase-3, caspase-7 and poly ADP-ribose polymerase (PRAP) in the cells.Tanshindiol C significantly decreases the levels of H3K27me3 in cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. RT-PCRCell Line: RAW264.7 cells Concentration: 1 μM, 3 μM, 10 μM Incubation Time: 24 h Result: Upregulated the Nrf2 and Prdx1 mRNA levels. Western Blot AnalysisCell Line: Mouse peritoneal macrophages Concentration: 1 μM, 3 μM, 10 μM Incubation Time: 24 h Result: Activated Nrf2 and upregulated Prdx1 expression in macrophages. Cell Cycle AnalysisCell Line: Pfeiffer cells Concentration: 1 μM, 2.5 μM and 5 μM Incubation Time: 72 hours Result: Induced accumulation of Pfeiffer cells in sub-G1 phase. Western Blot AnalysisCell Line: Pfeiffer cells Concentration: 1 μM, 3 μM Incubation Time: 72 hours Result: The levels of H3K27me3 was significantly decreased in the cells.

Form:Solid

IC50& Target:EZH2|0.55 μM (IC|50|)|SIRT1

Specifications

사양 및 순도

≥98%

생화학 및 생리적 메커니즘

Tanshindiol C is a S-adenosylmethionine-competitive EZH2 ( Histone Methyltransferase ) inhibitor with an IC 50 of 0.55 μM for inhibiting the methyltransferase activity. Tanshindiol C is also an activator of both Nrf2 and Sirtuin 1 (Sirt1) in macrophages.

보관 조건

Protected from light,Store at -80°C

배송

Dry ice packs + Cold packs

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순수함

≥98%

이름과 식별자

정식 스마일

CC1=COC2=C1C(=O)C(=O)C3=C2C=CC4=C3CC[C@H]([C@]4(C)O)O

이성체 SMILES

CC1=COC2=C1C(=O)C(=O)C3=C2C=CC4=C3CC[C@H]([C@]4(C)O)O

PubChem CID

126072

분자량

312.32

관련 대상(인간)

Daudi (625 Activities)

Explore this target

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

PC-3 (62116 Activities)

Explore this target

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

T98G (1524 Activities)

Explore this target

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

A549 (127892 Activities)

Explore this target

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

EZH2 Tclin Histone-lysine N-methyltransferase EZH2 (2012 Activities)

Discover Target: EZH2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

EZH2 Tclin EZH2/SUZ12/EED complex (20 Activities)

Discover Target: EZH2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

EZH2 Tclin EZH2/SUZ12/EED/RBBP7/RBBP4 (11 Activities)

Discover Target: EZH2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

작용 메커니즘

화학 및 물리적 특성

• 분자량: 312.300 g/mol
• XLogP3: 1.200
• Hydrogen Bond Donor Count: 2
• Hydrogen Bond Acceptor Count: 5
• Rotatable Bond Count: 0
• Exact Mass: 312.1 Da
• Monoisotopic Mass: 312.1 Da
• Topological Polar Surface Area: 87.700 Ų
• Heavy Atom Count: 23
• Formal Charge: 0
• Complexity: 546.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 2
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

자주 묻는 질문

What is the purity of this product?

This product is supplied at ≥98% purity (chemical assay). Lot-specific values are stated on the Certificate of Analysis.

How should this product be stored?

Store at ?80 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 97465-71-9, the molecular formula is C18H16O5, and the molecular weight is 312.32 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.