Tissue Single-cell Enzymatic Digestion Stop Solution - BioReagent

Cat. No.: T1508753

GRADE & PURITY BioReagent

Synonyms

温和终止液 | Dissociation Stop Solution | Digestion Stop Solution | Dissociation Stop Buffer | Enzyme Inhibitor Buffer | Single-Cell Digestion Termination Solution | Stop Buffer for Tissue Dissociation | Digestion Termination Solution

Storage

Store at 2-8°C

Shipped In

Wet ice

Application

Cell Culture, Cell Separation

개요

This product is used to terminate enzymatic digestion reactions of tissues or cells, rapidly neutralize enzyme activity, and protect cell integrity. It is widely used in the preparation of single‑cell suspensions.It can stop enzymatic digestion of various animal organs (including brain, liver, spleen, kidney, lung, tumor tissue, etc.) mediated by tissue single‑cell dissociation enzymes, thereby protecting tissue cells from damage caused by excessive enzymatic digestion.This greatly improves the success rate and data quality of downstream experiments such as flow cytometry, single‑cell sequencing, and cell culture.

Protocol

1. After completion of enzymatic digestion, add an equal volume or 2× volume of Stop Solution to the digestion system immediately (adjust the volume according to enzyme concentration and reaction conditions).
2. Mix gently and incubate at room temperature for 1–2 minutes to fully terminate enzyme activity.
3. Centrifuge at 300–400 ×g for 5 minutes to collect cells; discard the supernatant.
4. Resuspend the cell pellet in pre‑chilled PBS or medium for downstream experiments including flow cytometry, single‑cell sequencing, and cell culture.

Notes

1. Mix the Stop Solution thoroughly before use. If precipitation occurs, centrifuge and use the supernatant.
2. Enzymatic digestion conditions vary among tissue types. Pre‑experiments are recommended to optimize the volume of Stop Solution and reaction time.
3. This product is sterile. Use aseptic technique to avoid contamination.
4. For research use only.

Specifications

동의어

温和终止液 | Dissociation Stop Solution | Digestion Stop Solution | Dissociation Stop Buffer | Enzyme Inhibitor Buffer | Single-Cell Digestion Termination Solution | Stop Buffer for Tissue Dissociation | Digestion Termination Solution

사양 및 순도

BioReagent

안정성 및 스토리지

Store at 2-8℃ long term (12 months).

보관 조건

Store at 2-8°C

배송

Wet ice

이 제품은 콜드 체인 배송이 필요합니다.지상 및 기타 경제 서비스는 사용할 수 없습니다.

등급

BioReagent

자주 묻는 질문

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.