TNF alpha Mouse mAb - 1차 항체, specific to Human TNF alpha

Cat. No.: Ab215301
주문 가능
GRADE & PURITY Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) 1.0 mg/mL
Application
Detection Antibody, ELISA
Host species
마우스
Reactive species
인간
Isotype
Mouse IgG1
Clone Number
C08/3E2
Conjugation
Unconjugated
★
Size
USA
독일 (EU)*
Price
Qty
10μg
Ab215301-10μg
4 재고 있음
—
US$75.90
50μg
Ab215301-50μg
3 재고 있음
—
US$223.90
100μg
Ab215301-100μg
2 재고 있음
—
US$359.90
1mg
Ab215301-1mg
US 주문제작 · 2–4주 ·
—
US$1,023.90
Enter a quantity for the sizes you want to add.
🧪
Why this grade

Animal Free,Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL Animal Free,Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

개요

Mouse anti Human TNF alpha Antibody, clone C08/3E2, could be used for a Detection Antibody in sandwich ELISAs

Specifications

Product Name
TNF alpha Mouse mAb - 1차 항체, specific to Human TNF alpha
동의어
APC1 antibody | APC1 protein antibody | Cachectin antibody | DIF antibody | Differentiation inducing factor antibody | Macrophage cytotoxic factor antibody | Tnf antibody | TNF superfamily member 2 antibody | TNF superfamily, member 2 antibody | TNF, macr
사양 및 순도
Animal Free,Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL
생화학 및 생리적 메커니즘
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is i
숙주 종
마우스
특이성
Human TNF alpha
면역원
Recombinant protein corresponding to Human TNF alpha
변화
Unconjugated
등급
Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
라이트 체인 유형
kappa
SDS-PAGE
150 kDa
정화 방법
Protein A purified
일치하는 쌍
[Ab156547] TNF alpha Mouse mAb, Capture Antibody
집중력
1.0 mg/mL
보관 및 배송
보관 조건
Store at -20°C,Avoid repeated freezing and thawing
배송
Ice chest + Ice pads
안정성 및 스토리지
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
이미지

Sandwich ELISA - TNF alpha standard curve
Sandwich ELISA detection of Recombinant Human TNF-α Protein (rp156007) using TNF alpha Mouse mAb (Ab156547) as capture antibody at concentration of 1.0 µg/mL. Biotinylated TNF alpha Mouse mAb (Ab215301) as detection antibody at concentration of 0.5 µg/mL. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Detection range: 0.97 ng/mL - 1000.0 ng/mL

Indirect ELISA - TNF alpha standard curve
Immobilized Recombinant Human TNF-α Protein (rp156007) at 1.0 µg/mL  overnight at 4℃. Coated protein probed with TNF alpha Mouse mAb (Ab156547). Goat Anti-Mouse IgG H&L (HRP) (Ab156255) detected bound primary antibody.
EC50: 59.41 ng/mL

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

관련 대상(인간)
TNF Tclin Tumor necrosis factor (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
인증서(CoA, COO, BSE/TSE 및 분석 차트)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
자주 묻는 질문과 기사
솔루션 계산기
리뷰

고객 리뷰

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Animal Free grade guide → View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide → View PBS Only grade guide →

관련 상품

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Infliximab (anti-TNF-alpha) - 1차 항체, for ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) various applications, specific to TNFSF2/TNFa, >95%, Human IgG1, INHIBITOR of TNF-alpha inhibitor
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Goat Anti-Mouse IgG H&L (TRITC) - 2차 항체
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Goat Anti-Mouse IgG H&L (Cy5) - 2차 항체
Goat Anti-Mouse IgG H&L (HRP) - 2차 항체
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Rabbit Anti-Mouse IgG (HRP) - 2차 항체
Rabbit Anti-Mouse IgG (Biotin) - 2차 항체
Goat Anti-Mouse IgG H&L (HRP) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (TRITC) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (Biotin) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (FITC) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (Cy5) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (PE) - 2차 항체, Goat IgG
Goat Anti-Mouse IgG H&L (APC) - 2차 항체, Goat IgG
Certolizumab (anti-TNFa) - 1차 항체, specific to TNF, Human IgG1
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Goat Anti-Mouse IgG H&L (AF700) - 2차 항체, specific to Mouse IgG, Goat IgG
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TNF alpha Mouse mAb - 1차 항체, specific to Human TNF alpha
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Goat Anti-Mouse IgG H&L (PE-Cy7) - 2차 항체
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Goat Anti-Mouse IgG H&L (AF546) - 2차 항체
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