BioReagent, HEPES-free BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
alanced Salt Solution (BSS) matches the pH value, osmotic pressure and other environmental conditions under cell‑growth status. It functions to maintain osmotic pressure, regulate acid‑base balance, and supply energy and inorganic salts essential for cell survival and metabolism, meeting the basic requirements for cell survival and certain metabolic activities in in‑vitro experiments.
Tyrode's Solution powder is a type of balanced salt solution. It is mainly composed of sodium chloride, phosphate, calcium chloride, glucose, etc., and contains no HEPES. Its buffering capacity is slightly weaker than modified Tyrode’s solution powder (Aladdin Cat. No.: M1529492). In this product, calcium chloride is stored separately from other components. Dissolve them respectively and mix before use to prevent insoluble precipitates caused by simultaneous dissolution. It is frequently applied for mammalian organ perfusion, tissue rinsing, and maintaining normal physiological functions of isolated intestinal muscle.
This reagent is for research use only and shall not be used for clinical diagnosis or other purposes.
Contents & Storage
| Component No. | Component Name | Appearance | 1L | Storage |
| T1529491A | Calcium-free Tyrode's solution powder | Powder | 1EA | 2-8℃. |
| T1529491B | Calcium salt solution | Liquid | 1EA | 2-8℃. |
Operating Procedures (For reference only):
1. For 1‑L‑size Calcium-free Tyrode's solution powder, fully dissolve the powder in 800 mL deionized water in a 1‑L beaker. Add calcium salt solution, mix well and make up the volume to 1000 mL for preservation or rinsing of isolated tissues.
2. pH adjustment is generally unnecessary; adjust pH manually if required. Valid for 6 months when stored at 4 °C.
Precautions:
1. Precipitates are prone to form due to calcium ions in calcium salt solution. Minor precipitates can be dissolved by heating or removed by filtration before use. Discard the solution in case of massive precipitates.
2. This product contains glucose. Bacterial growth may occur if the prepared solution is improperly stored. Check solution status prior to use.
Comprehensive hazard, handling, storage, and regulatory compliance document.
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