UCP2 Antibody - 1차 항체, specific to UCP2, Rabbit IgG

Cat. No.: Ab133399

GRADE & PURITY Carrier Free, ExactAb™, Validated, 2.1 mg/mL

Application

IHC, WB

Host species

토끼

Reactive species

마우스, 인간

Isotype

Rabbit IgG

Conjugation

Unconjugated

개요

Rabbit anti Human UCP2 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function
UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.

Specifications

Product Name

UCP2 Antibody - 1차 항체, specific to UCP2, Rabbit IgG

동의어

BMIQ4 antibody | Mitochondrial uncoupling protein 2 antibody | SLC25A8 antibody | Solute carrier family 25 member 8 antibody | UCP 2 antibody | ucp2 antibody | UCP2_HUMAN antibody | UCPH antibody | Uncoupling protein 2 antibody | Uncoupling protein 2 mito

사양 및 순도

ExactAb™, Validated, Carrier Free, 2.1 mg/mL

숙주 종

토끼

특이성

UCP2

면역원

Fusion protein of human UCP2 (AA 134-170)

포지티브 제어

WB: HeLa, HT-29 and LNCaP cell lysates. IHC: Human liver cancer tissue.

변화

Unconjugated

등급

Carrier Free, ExactAb™, Validated

아이소타입

Rabbit IgG

SDS-PAGE

150 kDa

정화 방법

Protein A purified

집중력

2.1 mg/mL

Product Line

생물 제품

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: UCP2 Antibody (Ab133399)

UCP2 Antibody (Ab133399) - Western Blot
All lanes: UCP2 Antibody (Ab133399) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 33 kDa
Observed band size: 30 kDa
Exposure time: 19.7 s 

IHC: UCP2 Antibody (Ab133399)

UCP2 Antibody (Ab133399) - IHC
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using UCP2 Antibody (Ab133399). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

적용

적용

Dilution info

WB

1/500-1/1000

IHC

1/50 - 1/100

관련 대상(인간)

UCP2 Tbio Mitochondrial uncoupling protein 2 (0 Activities)

Discover Target: UCP2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

자주 묻는 질문

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (UCP2 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.