등급 & 순도Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.>150 U/mg protein; expressed in E.coli
EnzymoPure™, ActiBioPure™, Recombinant, Bioactive, High Performance, >150 U/mg protein; expressed in E.coli ActiBioPure™,Bioactive,High Performance,Recombinant,EnzymoPure™ 최소한의 기준선 간섭이 필요한 민감한 크로마토그래피 및 분석 워크플로우용.
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보관 및 배송
Store at -20°C,Avoid repeated freezing and thawing Ice chest + Ice pads 출고. 정확한 사양은 로트별 COA를 확인하세요.
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품질 문서
SDS, COA, 데이터시트, 사양서를 다운로드할 수 있습니다. 로트별 COA는 로트 번호 조회를 통해 확인할 수 있습니다.
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문헌적 근거
크로마토그래피, 유기 합성 및 교차 커플링 반응 분야의 동료 심사 논문 45편에서 인용되었습니다.
개요
Ureases, functionally, belong to the superfamily of amidohydrolases and phosphotriesterases. It is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Reaction Equation Specification Specific Activity: >150 U/mg protein Contaminants: NADPH oxidase: <0.001% Properties pH stability: pH 8.0-9.5 (37°C, 1 weeks) Thermal stability: ≤ 65°C (pH 8.0, 10 min.) Optimum pH: 6.0 Optimum temp.: ≥37°C Km value: 1.94× 10⁻⁵ mmol/L (Urea) Molecular weight: 60.3 kDa α subunit, 11.7 kDa β subunit, 11.1 kDa λ subunit (SDS-PAGE) Assay Procedure I Spectrophotometric Method Wavelength: 340 nm, Light path length: 1 cm Temperature: 25°C Pipette the following reagents into a cuvette: 3.00 mL: Triethanolamine-HCl buffer (0.1 mol/L, pH 7.0) containing Urea (1 mol/L)、α-Ketoglutarate (5 mmol/L)、NADPH (0.24 mmol/L)、GIDH (20 U/mL) 0.02 mL: rUrease (approx. 1.5 U/mL) II Calculation ΔA/min: The change in absorbance at 340 nm per minute V: Total volume of reaction mixture (3.02 mL) D: Enzyme dilution factor 6.2: mmol/L extinction coefficient of NADPH (L⋅mmol −1⋅cm −1) d: Light path length (1 cm) v: Volume of enzyme sample (0.02 mL) Reference Data
사양
Product Name
Urease (URH), CAS No.9002-13-5
동의어
ureA | ureB | ureC
등급
ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™
사양 및 순도
EnzymoPure™, ActiBioPure™, Recombinant, Bioactive, High Performance, >150 U/mg protein; expressed in E.coli
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자주 묻는 질문
What does Bioactive mean?
Bioactive indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.
What does Recombinant mean?
Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What are the CAS number, molecular formula and molecular weight?
The CAS Number is 9002-13-5.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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