This product is 2× YT Broth Medium. Compared with the classic formula, the concentrations of tryptone and yeast extract are doubled for richer nutrition. It can significantly increase the cell density of Escherichia coli and is an excellent choice for recombinant plasmid amplification and recombinant protein induction and expression.
Key Advantages
• Double nutrition for efficient propagation: Contains 16 g/L tryptone + 10 g/L yeast extract, providing abundant growth factors for recombinant E. coli (e.g., BL21, Rosetta) to readily meet high-density fermentation requirements.
• Classic formula with broad compatibility: 5 g/L sodium chloride maintains suitable osmotic pressure, fully compatible with various antibiotic selections (e.g., Amp, Kana), suitable for culture of diverse recombinant strains.
• Ready-to-use powder for easy operation: Weigh and dissolve as required to reduce errors from complicated preparation.
Medium Formula (per liter)：
Component | Content |
Tryptone | 16.0g |
Yeast Extract | 10.0g |
Sodium Chloride | 5.0g |
Instructions for Use:
Weigh 31.0 g of the product into 1 L of distilled or deionized water (the preparation volume can be increased or decreased proportionally), heat to boiling until completely dissolved, dispense, autoclave at 121 °C for 15 minutes, cool and set aside for use.
Precautions:
1. pH 7.0±0.2 at 25 °C.
2. Avoid direct contact with skin and eyes during handling. In case of accidental contact, rinse immediately with plenty of clean water.
3. This product is for laboratory research only and is not intended for clinical diagnosis.
