Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA Azide Free,Carrier Free,ExactAb™,PBS Only,Validated para fluxos de trabalho cromatográficos e analíticos sensíveis que exigem interferência mínima da linha de base.

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Armazenamento e envio

Store at 2-8°C,Do not freeze Envio Wet ice,Do not freeze. Consulte o COA específico do lote para obter as especificações exatas.

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Documentos de qualidade

SDS, COA, ficha técnica e folha de especificações disponíveis para download. COA específico do lote acessível por meio de pesquisa por número de lote.

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Comprovação documental

Citado em 0 publicações revisadas por pares nas áreas de cromatografia, síntese orgânica e reações de acoplamento cruzado.

Visão geral

Mouse anti Human EGFR Antibody, Monoclonal (528), could be used for in vitro EGFR blockade, in vivo EGFR blockade in xenografts, WB, Functional assays, IP, IHC, ICC, IF, Flow and so on.

Application:

in vitro EGFR blockade: Use at an assay dependent concentration.

in vivo EGFR blockade in xenografts: Use at an assay dependent concentration.

WB: Use at an assay dependent concentration.

Functional assays: Use at an assay dependent concentration.

IP: Use at an assay dependent concentration.

IHC: Use at an assay dependent concentration.

ICC/IF: Use at an assay dependent concentration.

Flow: Use at an assay dependent concentration.
Protein Function
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action.

Especificações

Product Name

EGFR Mouse mAb - Anticorpo primário, specific to EGFR, Mouse IgG2a

Sinónimos

Especificações e pureza

Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA

Espécie hospedeira

Rato

Especificidade

EGFR

Imunogénio

Purified EGFR from A431 cells

Conjugação

Unconjugated

Grau

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isótipo

Mouse IgG2a

Tipo de corrente de luz

kappa

SDS-PAGE

150 kDa

Método de purificação

Protein A purified

Concentração

See COA

Product Line

Produtos biológicos

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at 2-8°C,Do not freeze

Enviado em

Wet ice,Do not freeze

Estabilidade e armazenamento

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon receipt, it is recommended to aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

Imagens

Flow Cytometry: EGFR Mouse mAb (Ab101169)

EGFR Mouse mAb (Ab101169) - Flow Cytometry
Flow Cytometry analysis of A431 cells labelling EGFR (red) with EGFR Mouse mAb (Ab101169) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicação

Dilution info