Recombinant EGFR Antibody (Biotin) - Primary antibody, specific to EGFR, Human IgG2SA

Cat. No.: Ab176025

GRADE & PURITY Recombinant, Validated, ExactAb™, Azide Free, 1.0 mg/mL

Application

Flow Cytometry

Host species

Human

Reactive species

Human

Isotype

Human IgG2SA

Conjugation

Biotin

Overview

Application:
Flow: ≤1.0μg/test
For flow cytometric staining, the suggested use of this reagent is ≤1.0μg per million cells in 100 µL staining volume.

Specifications

Product Name

Recombinant EGFR Antibody (Biotin) - Primary antibody, specific to EGFR, Human IgG2SA

Synonyms

EC 2.7.10.1 | Proto-oncogene c-ErbB-1 | Receptor tyrosine-protein kinase erbB-1 | Cell growth inhibiting protein 40 antibody | Cell proliferation inducing protein 61 antibody | EGF R antibody | EGFR antibody | EGFR_HUMAN antibody | Epidermal growth factor

Specifications & Purity

ExactAb™, Recombinant, Validated, Azide Free, 1.0 mg/mL

Host species

Human

Specificity

EGFR

Positive Control

Flow: A431

Conjugation

Biotin

Grade

Azide Free, ExactAb™, Recombinant, Validated

Isotype

Human IgG2SA

Light Chain Type

kappa

Purification Method

Protein A purified

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: Recombinant EGFR Antibody (Biotin) (Ab176025)

Recombinant EGFR Antibody (Biotin) (Ab176025) - Flow Cytometry
Flow cytometry analysis of EGFR expression by A431 cells (red) compared to an isotype control (blue). Cells were stained with Recombinant EGFR Antibody (Biotin) (Ab176025) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤1.0μg/ test

  For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

EGFR Tclin Epidermal growth factor receptor (0 Activities)

Discover Target: EGFR

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

EGFR Tclin Epidermal growth factor receptor erbB1 (33727 Activities)

Discover Target: EGFR

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

EGFR Tclin MER intracellular domain/EGFR extracellular domain chimera (2 Activities)

Discover Target: EGFR

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

EGFR Tclin Protein cereblon/Epidermal growth factor receptor (191 Activities)

Discover Target: EGFR

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

EGFR Tclin EGFR/PPP1CA (1 Activities)

Discover Target: EGFR

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

EGFR Tclin VHL/EGFR (323 Activities)

Discover Target: EGFR

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action