Determination of pharmacokinetic parameters
Sulfonamides containing p-aminobenzene in their structure can react diazotically with sodium nitrite in an acidic environment to produce a diazonium salt. This
Topic
Pharmacognosy
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
75 resources · protocols, FAQs & articles
designed experiment
Through the creative design of a functional animal experiment (including pathological models of animals), within certain experimental conditions and scope, comp
Experiments for the determination of blood concentration and half-life of drugs
Measurement of blood concentration and half-life of drugs is used (1) in order to guide the clinical selection of the optimal therapeutic regimen and the most a
Preparation, activity determination and application of thin-layer plates
Thin layer chromatography (TLC) is mainly used in the study of chemical composition of natural medicines for the pre-testing of chemical components, identificat
Experiments on the isolation and identification of monocotyledonine
Euphorbiaceae plant a leaf autumn, also known as the bottom of the leaf beads, also known as dog apricot strip, its roots, leaves and shoots contain a variety o
Experimental effects of drugs of the efferent nervous system on blood pressure in rabbits
Learning the method of acute blood pressure experiment in anesthetized animals; observing the effect of efferent nerve drugs on the blood pressure of animals, d
Membrane agent inspection test
Membrane (Appendix I M of the 2010 edition of the Chinese Pharmacopoeia, Part II) refers to the membrane preparation made by processing the drug and suitable fi
Implant checking experiments
Implants (Appendix I J of the 2010 edition of the Chinese Pharmacopoeia, Part II) are sterile solid preparations of drugs and excipients for implantation in the
Capsule inspection test
Capsule (Chinese Pharmacopoeia, 2010 Edition, Part II, Appendix E) refers to solid preparations in which the drug or excipients are filled in hollow capsules or
Suppository Inspection Laboratory
Suppositories (Chinese Pharmacopoeia, 2010 Edition, Part II, Appendix I D) refer to solid preparations made of drugs and suitable matrices for cavernous adminis
Experiments on the preparation of liquid pharmaceuticals classified by route of administration and method of application
In clinical work, due to the different medical effects and purposes of medication, liquid pharmaceuticals are often classified according to the routes of admini
Experiments on the preparation of emulsion-type liquid pharmaceuticals
Emulsion-type liquid pharmaceuticals, also known as emulsions, refers to the two immiscible liquids are mixed, one of which is dispersed in the liquid droplet s
Practice experiments for weighing operations
The experimental objectives of this experiment are: 1. to familiarize with the structure and performance of the rack-and-pan balance and electronic balance, and
Access to the Chinese Pharmacopoeia
The objectives of this experiment are: 1. to familiarize with the requirements of the laboratory; 2. to familiarize with and manage the basic laboratory apparat
Pre-testing of chemical constituents of natural medicines
The chemical components contained in natural medicines are relatively complex, and for natural medicines of unknown composition, before extracting and separatin
Activity Screening and Activity Tracking Experiments of Traditional Chinese Medicines
DPPH (2,2-diphenyl-1-picrylhydrazyl radical, 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl) is a relatively stable lipid radical with a free electron on its N.
Extraction, isolation and characterization of the alkaloids of Hantahexaenia japonica
Han Fangji is the root of Stephania tetrandra S. Mcore (防己科千金藤属物), an expectorant, antipyretic and analgesic drug whose active ingredients are alkaloids
Toxic reactions to streptomycin and antidote experiments
Streptomycin is an aminoglycoside antibiotic, and its acute toxic reaction is neuromuscular blockage, with weakness of limbs and even respiratory depression. In
Experimental action of cardiac glycosides on isolated frog hearts
After the frog's heart is isolated, a frog heart cannula containing Ren's fluid is inserted into the ventricle, and this artificial perfusion method is used to
Experiments on the action of efferent nervous system drugs on isolated intestinal muscles
The isolated intestinal muscle of animals still has the characteristics of excitation and contraction in a suitable environment of nutrient solution. Since ther
Experimental effects of efferent nervous system drugs on blood pressure in anesthetized dogs
Using a direct blood pressure measurement method, an arterial cannula inserted into the common carotid artery and a pressure transducer form an anticoagulant cl
Tincture checking experiments
Tincture ("Chinese Pharmacopoeia" 2010 version of the second part of Appendix I C) refers to the drug with a prescribed concentration of ethanol leaching or dis
Experiments on the effect of neostigmine on the muscarinic action of succinylcholine and cylindrolysine
Myorelaxants are divided into two types: depolarizing and non-depolarizing. Succinylcholine is the representative drug of the depolarizing type of muscarinic dr
Experiments on the preparation of suspension-type liquid pharmaceuticals
Suspension liquid pharmaceuticals refers to the insoluble solid drugs to the particle state dispersed in the liquid dispersion medium formed by the non-homogene
Experimental methods for the determination of blood concentration and calculation of pharmacokinetic parameters of sulfonamides
Sulfonamides and compounds with free amino groups on the benzene ring undergo diazotization with sodium nitrite in acidic solutions to produce diazonium salts,
Tests for syrup preparations
Syrup (Appendix I K of Part II of the 2010 edition of the Chinese Pharmacopoeia) refers to a concentrated aqueous sucrose solution containing the drug for oral
Experimental effects of sodium phenobarbital and actinomycin D on cytochrome P-450 content in mouse liver
P-450 belongs to hemoglobin protein, and its reduced form combines with CO and shows an absorption peak at 450 nm. Therefore, the content of P-450 can be determ
Experiments on the effect of dose on drug action
The purpose of this experiment was to observe the differences in the effects of different doses of sodium pentobarbital on mice. After the administration of the
Experimental effects of drugs on spontaneous activity in mice
Spontaneous activity is a physiological characteristic of normal animals. The amount of spontaneous activity often reflects the state of central excitation or i
Physicochemical and microscopic identification experiments of Rheum palmatum
The purpose of this experiment is to master the characteristics of raw drug properties of Rheum palmatum; to master the cross-sectional tissue characteristics a
Chinese medicine composition analysis and structure identification experiment
The objectives of this experiment are: 1. to master the basic principles and operation methods of high performance liquid chromatography (HPLC). 2. to master th
Observation experiment on the diuretic effect of drugs
NA is an a receptor agonist, a larger dose can constrict renal blood vessels, reduce renal blood flow, resulting in a decrease in urine output; furosemide is a
Experimental prevention of barium chloride-induced arrhythmias by propranolol and quinidine
Barium chloride induces arrhythmias by increasing the inward Na+ current in Purkinje fibers and increasing the depolarization rate of the maximal diastolic pote
Applying, coating, filming agent inspection test
Application (Appendix I T of Part II of the 2010 edition of the Chinese Pharmacopoeia) refers to liquid preparations containing a solution, emulsion or suspensi
An experiment on the effect of caffeine on classmates' numeracy and heart rate
The purpose of this experiment is to master the basic process of T-test, a statistical method of measuring information, and to familiarize with the effect of ca
Experimental determination of LD50
The lethal dose (LD50) is an indication of the level of toxicity of drugs, poisons and pathogenic microorganisms. It represents the dose or concentration that k
Experiments on the observation of the inhibitory and excitatory effects of drugs on respiration
High-dose injections of aminoglycoside antibiotics can produce a blockade of the muscle junction, which is manifested by weakness of the extremities. Dyspnea or
Experimental effects of drugs on blood pressure and heart rhythm in rabbits
Epinephrine alone has a pressor effect, while alpha-blockers can reverse its pressor effect. Barium chloride can promote the inward flow of sodium ions in the P
Experimental determination of plasma half-life and apparent volume of distribution of drugs
In pharmacology experiments, there are many factors affecting the experimental results, some of which can be controlled, while some are difficult to control. In
Experiments on the effect of different routes of administration on drug action
In pharmacology experiments, there are many factors affecting the experimental results, some of which can be controlled, while some are difficult to control. In
Experiments on the relationship between solubility and action of barium salts
Solubility-action relationship experiments with barium salts can be used to study the effect of the physicochemical properties of a drug on its action.
Analytical experiments on the mechanism of action of unknown antihypertensive drugs
Ganglion blockers affect the blood pressure drop induced by direct stimulation of the vagus nerve and the boosting effect of clamping the common carotid artery,
Experimental effects of efferent nervous system drugs on blood pressure and hemodynamics
Efferent nervous system drugs produce cardiovascular effects by acting on the corresponding receptors on cardiac and vascular smooth muscle, resulting in corres
Detergents, rinses, and enemas screening tests
Lotion (Chinese Pharmacopoeia, 2010 Edition, Part II, Appendix I S) means a solution, emulsion, or suspension containing a drug for cleansing or applying to unb
Experimental measurement of pharmacokinetic parameters of sulfonamides
Sulfonamides can react with certain reagents to form colored substances, and their blood concentrations can be quantified by colorimetry. In acidic environments
Determination of ED50 in mice by intraperitoneal injection of cartridge arrow toxins
A quantitative response is the intensity of the response in an individual and can be expressed as a continuous value, e.g., blood pressure, urine output, etc. T
Experimental action of prazosin on isolated vascular smooth muscle
The rabbit isolated aortic strip assay can be used to observe the effects of drugs on the diastolic and contractile functions of vascular smooth muscle. Observa
Experiments on the action of drugs on isolated intestinal tubes
The purpose of this experiment is to master the experimental conditions of isolated intestinal smooth muscle organ and the effects of drugs on isolated intestin
Compound iodine oral solution preparation experiment
This experimental method was obtained from the official website of the Fourth Military Medical University
Experiments on the preparation of cresol soap solution (Lysol, coal phenol soap solution)
This experimental method was obtained from the official website of the Fourth Military Medical University
Orthogonal experiments to optimize the extraction of tanshinone ⅡA
Tanshinone is a fat-soluble diterpenoid compound with orange-yellow and orange-red features in Salvia miltiorrhiza, which can be classified into more than 10 ki
Experiment on the preparation of glycerite lotion
This experimental method was obtained from the official website of the Fourth Military Medical University
Paper chromatography experiments on amino acids
This experiment was obtained from the Department of Physiology and Biochemistry, College of Plant Science and Technology, Bayi Agricultural and Reclamation Univ
Experiments on the basic properties of enzymes
Department of Physiology and Biochemistry, College of Plant Science and Technology, Heilongjiang Bayi Agricultural and Reclamation University, China
Enzyme specificity assay
Department of Physiology and Biochemistry, College of Plant Science and Technology, Heilongjiang Bayi Agricultural and Reclamation University, China
Cod liver oil emulsion preparation experiment
This experimental method was obtained from the official website of the Fourth Military Medical University
Competitive antagonism of acetylcholine by atropine and determination of pA2 value in experiments
The purpose of this experiment is to grasp the method of determining the pA2 value and its significance while also focusing on the observation of the competitiv
Experimental determination of the elimination rate constant of riboflavin tablets by the urine drug concentration method
This experimental method was obtained from the official website of the Fourth Military Medical University
Experiments on the effect of temperature on enzyme activity
Department of Physiology and Biochemistry, College of Plant Science and Technology, Heilongjiang Bayi Agricultural and Reclamation University, China
Experimental determination of pharmacokinetic parameters of sulfadiazine sodium in normal and renal failure rabbits
The purpose of this experiment is to master the replication method of acute renal failure rabbit model. To master the surgical procedure of arterial cannulation
Determination of pA2
Antagonism parameter (pAx): for competitive antagonism, when the agonist and antagonist combined, X times the concentration of agonist effect produced exactly e
Experimental anticonvulsant action of phenobarbitalina
This experiment is from the official website of Zhengzhou University School of Medicine
Aminophylline injection blood drug concentration measurement experiment
This experimental method was obtained from the official website of the Fourth Military Medical University
Experiments on identification of stem, wood and skin Chinese medicines
The purpose of this experiment is to master the mulberry, mistletoe, mouton, dahuangteng, sumac, chickweed, Tongzhi, hook vine, cinnamon, eucommia, Xiangjiappi,
Aspirin preparation experiments
This experimental method was obtained from the official website of the Fourth Military Medical University
Extraction and isolation experiments of rutinosides
This experimental method was obtained from the official website of the Fourth Military Medical University
Experiments on Identification of Proprietary Chinese Medicines
The purpose of this experiment is to master the wolfberry chrysanthemum dihuang pill, heavenly king tonic pill traits identification characteristics; master the
Laboratory for identification of animal and mineral Chinese medicines
The purpose of this experiment is to master the traits and identification characteristics of the herbs, such as stone diamine, pearl, dillon, earthworm, money w
Experiments on the identification of whole herbs, mushrooms, resins and other types of Chinese medicines
The purpose of this experiment is to master ephedra, money grass, fine Xin, mint, andrographis paniculata, Poria, poria, frankincense, myrrh, amber, Tianzhuhuan
Fruit and Seed Traditional Chinese Medicine Identification Laboratory
The purpose of this experiment is to master the Schisandra chinensis, bitter almonds, peach kernel, make gentleman, cumin, forsythia, cuscuta, strychnine, betel
Leafy and floral Chinese medicine identification experiment
The purpose of this experiment is to master the lateral cypress leaves, Polygonum tinctorium leaves, leaves, senna leaves, leaves, leaves, leaves, pine pollen,
Roots and rhizomes Chinese medicine identification experiment
The objectives and requirements of this experiment are to master the traits of Mianma Guanzhong, rhubarb, He Shouwu, hyssop, Sichuan hyssop, Shanglu, white peon
Experiments on the effect of toxaphene on the action of acetylcholine
The purpose of this experiment was to learn the experimental conditions for studying the quantitative and qualitative effects of drugs. To observe the effects o
calcium and magnesium antagonism test
The purpose of this experiment is to master the principle of action of calcium and magnesium and its specific antagonistic relationship and to understand its pr
Vitamin C Injection Stability Test
1. Stability of pharmaceutical preparations The basic requirements for pharmaceutical preparations should be safety, efficacy and stability. Stability refers to
Related topics
Techniques often explored alongside immunological experiments.