Experimental prevention of barium chloride-induced arrhythmias by propranolol and quinidine
Experimental prevention of barium chloride-induced arrhythmias by propranolol and quinidine
Barium chloride induces arrhythmias by increasing the inward Na+ current in Purkinje fibers and increasing the depolarization rate of the maximal diastolic potential. The purpose of this experiment was to observe the preventive effect of propranolol and quinidine on barium chloride-induced arrhythmias and to understand the method of barium chloride-induced arrhythmias.
Operation method
intravenous injection
Principle
Barium chloride induces arrhythmias by increasing the inward Na+ current in Purkinje fibers and increasing the rate of depolarization of the maximal diastolic potential.
Materials and Instruments
Rats Move 1. 3 rats, weighed and numbered. Caveat 1. Barium chloride needs to be freshly prepared and administered rapidly intravenously. 2. propranolol and quinidine should be administered slowly intravenously. 3. rapid administration of barium chloride results in arrhythmias in most animals during or 30 seconds after administration. 4. chloral hydrate can sometimes induce arrhythmias. 4. Chloral hydrate may sometimes induce arrhythmias. For more product details, please visit Aladdin Scientific website.
Chloral hydrate, barium chloride, propranolol, quinidine, saline.
Powerlab electrocardiographic recording equipment set Rat table Surgical instruments set Gauze Cotton balls
2. 10% chloral hydrate (3-4 ml/kg) was injected intraperitoneally for anesthesia, and the rats were fixed in dorsal position on the operating table, and the femoral vein was cannulated for drug administration.
3. Ⅱ lead electrocardiogram was recorded.
4. Two rats were injected intravenously with 0.1% propranolol (2 ml/kg) and 0.5% quinidine (2 ml/kg), and the other was injected intravenously with an equal volume of saline as a control. The electrocardiograms were recorded at 1, 3 and 5 minutes after administration.
5. 5 minutes later, 0.3% barium chloride (1 ml/kg) was injected intravenously and the ECG was recorded immediately, followed by ECGs at 15 and 30 seconds and at 1, 3, 5, 10, 15, 20 and 30 minutes after administration.