488-Phalloidin, CAS No.P378332

CAS: P378332 Cat. No.: P378332

Overview

Phalloidin CruzFluor™ 488 Conjugate is a green fluorescent phalloidin conjugate that is an excellent substitute for AlexFluor® 488. Phalloidin is a heptapeptide phallotoxin produced by|Amanita phalloides|, the death cap mushroom. Phalloidin binds and stabilizes filamentous F-actin, preventing the dissociation of paired filaments and disturbing the natural equilibrium between filamentous (polymeric) and globular (monomeric) actin in the cell, as well as inhibit the ATP hydrolysis activity of F-actin. Actin is used by the cell for mechanical processes such as growth, scaffolding of the cytoskeleton, and locomotion, and disruption of this actin equilibrium destroys cellular functioning. The magnitude by which the actin protein differs from species to species is no more than 20%.|These properties of Phalloidin make Phalloidin CruzFluor™ Conjugates a fantastic tool for labeling, identifying and quantitating F-actins. The Phalloidin CruzFluor™ Conjugates are used at nanomolar concentrations with cell-free experiments, cell culture experiments, formaldehyde-fixed, permeabilized tissue sections and the study of actin networks. Because Phalloidin CruzFluor™ Conjugates are offered in different colors the series is excellent for multicolor imaging applications.
Phalloidin CruzFluor™ conjugates can be used alongside antibodies in IF staining of formalin-fixed cells and formalin-fixed, frozen tissue sections. The Phalloidin will stain F-actin.1. Prepare 1000 X Phalloidin DMSO stock solution: by adding 30 uL of DMS
Phalloidin CruzFluor™ 488 Conjugateis a fluorescent phalloidin conjugate that selectively binds to F-actins

Specifications

Product Name

488-Phalloidin, CAS No.P378332

Synonyms

Phalloidin CruzFluor™ 488 Conjugate also known as Phalloidin-iFluor™ 488 Conjugate

CAS

P378332

Molecule Type

Peptide

Storage and Shipping

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads