ARRDC3 Antibody - Primary antibody, specific to ARRDC3, Rabbit IgG

Cat. No.: Ab089693

GRADE & PURITY Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Monkey, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human ARRDC3 Antibody, Polyclonal, could be used for WB, IHC and so on.

Specifications

Product Name

ARRDC3 Antibody - Primary antibody, specific to ARRDC3, Rabbit IgG

Synonyms

ARRDC 3 antibody | Arrestin domain containing 3 antibody | Arrestin domain containing protein 3antibody | KIAA1376antibody | TBP-2-like inducible membrane protein antibody | Thioredoxin binding protein 2 like inducible membraneantibody | TLIMPantibody | A

Specifications & Purity

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Biochemical and Physiological Mechanisms

Adapter protein that plays a role in regulating cell-surface expression of adrenergic receptors and probably also other G protein-coupled receptors (PubMed:20559325, PubMed:21982743, PubMed:23208550). Plays a role in NEDD4-mediated ubiquitination and endo

Host species

Rabbit

Specificity

ARRDC3

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ARRDC3.

Positive Control

WB: MCF7 cell lysate. IHC: Human brain tissue. IF/ICC: HeLa cells.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Immunogen affinity purified

Concentration

1.0 mg/mL

Product Line

biological_products

Storage and Shipping

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: ARRDC3 Antibody (Ab089693)

ARRDC3 Antibody (Ab089693) - Western Blot
All lanes: ARRDC3 Antibody (Ab089693) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 50 kDa
Exposure time: 2.9 s

IHC: ARRDC3 Antibody (Ab089693)

ARRDC3 Antibody (Ab089693) - IHC
Immunohistochemical analysis of ARRDC3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with ARRDC3 Antibody (Ab089693) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC: ARRDC3 Antibody (Ab089693)

ARRDC3 Antibody (Ab089693) - IF/ICC
IF analysis of ARRDC3 Antibody (Ab089693) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with ARRDC3 Antibody (Ab089693) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Application

Application

Dilution info

IF/ICC

1/200

WB

1/500-1/1000

IHC

1/50-1/100

Associated Targets(Human)

ARRDC3 Tbio Arrestin domain-containing protein 3 (0 Activities)

Discover Target: ARRDC3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

Item-specific tested applications and recommended dilutions are not provided in the Product Data. Refer to the CoA/Spec Sheet for authoritative guidance.

General starting points (literature/common practice; not validated for this item):

• Western blot (WB): Start at 1:1,000 for chemiluminescent detection; adjust 1:500–1:5,000 based on signal/background. Load lysate with verified ARRDC3 expression as positive control; include a knockout/siRNA control where possible.
• Immunofluorescence (IF/ICC): Begin at 1:200 in blocking buffer; optimize fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1–0.3% Triton X-100) as needed.
• Immunohistochemistry (IHC-P): Trial 1:200 after antigen retrieval (e.g., citrate pH 6.0 or Tris-EDTA pH 9.0); fine-tune retrieval and blocking conditions empirically.
• ELISA: Coating 0.5–2 µg/mL for capture; detection 0.1–1 µg/mL depending on substrate and instrument sensitivity.
• Immunoprecipitation (IP): 1–5 µg antibody per 500–1,000 µg lysate protein; optimize lysis buffer stringency to preserve ARRDC3–partner interactions.

Controls and validation:

• Include isotype controls and secondary-only controls as appropriate.
• Verify specificity using genetic controls (knockout/knockdown) or overexpression with epitope-tagged ARRDC3.

These are general guidelines; tailor to your system and consult the product CoA/Spec Sheet when available.

Biological Roles

Focus: ARRDC3 (Arrestin domain-containing protein 3), the antigen recognized by this antibody.

General biology (literature, not item-specific):

• Family: ARRDC3 is a member of the arrestin domain-containing (ARRDC) adaptor proteins related to β-arrestins.
• Cellular role: Functions as an adaptor in membrane protein trafficking, often linking cargo receptors to endocytic and ubiquitination machinery.
• Processes: Implicated in regulation of GPCR internalization and downregulation, interaction with E3 ubiquitin ligases, and coordination of endosomal sorting. May influence cellular signaling dynamics through receptor turnover and trafficking pathways.
• Localization: Predominantly cytoplasmic with membrane association depending on cargo/receptor engagement; can localize to endosomes (literature context).
• Experimental utility: Antibodies to ARRDC3 enable detection of protein expression, subcellular localization, and analysis of pathway modulation in cell and molecular biology studies.

Note: The above reflects general scientific literature and is provided for research context only. It does not constitute functional validation for this specific antibody lot. Consult the CoA/Spec Sheet for experimental validation data for SKU Ab089693.

Buffer Applications

Not applicable. This product is an antibody reagent, not a buffering agent or buffer system.

Practical note:

• Prepare and use standard immunoassay buffers (e.g., PBS or TBS with appropriate blocking agents and detergents) according to your application. Refer to Application Protocols and your institutional SOPs.

Green Alternatives

Not directly applicable. Antibody reagents are aqueous protein solutions rather than organic solvents or reagents commonly evaluated for green chemistry substitution.

Sustainability considerations (general laboratory practice):

• Minimize single-use plastics by planning aliquots efficiently.
• Use phosphate-free or Tris-based buffers responsibly to reduce environmental phosphate loading when appropriate.
• Consolidate cold-chain shipments where feasible; this product ships on ice packs per Product Data.

Pharmaceutical Uses

Not applicable. This product is designated for research use only and is not intended for human or veterinary therapeutic or diagnostic applications.

Formulation note (general for laboratory use):

• Carrier-free antibodies are suited for custom conjugation and assay development. Any downstream use in GMP or clinical workflows would require separate qualification and is outside the scope of this product.

Physical Properties

Item-specific physicochemical specifications are not provided for this antibody product.

Item-specific (from Product Data):

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

General/literature values for typical IgG-class antibodies (for context, not specifications):

• Typical molecular weight: ~150 kDa for full-length IgG; may vary for fragments or engineered formats.
• Isoelectric point (pI): Commonly ~6–9 depending on subclass and sequence.
• Solubility: Readily soluble in aqueous biological buffers (e.g., PBS) at mg/mL concentrations; avoid extreme pH or denaturants unless intended.
• Stability: Improved by low temperature storage, neutral pH buffers, and inclusion of stabilizers (e.g., glycerol, sugars, proteins) where appropriate to the application; this item is listed as carrier free, so no stabilizing proteins are included by default.
• UV absorbance: A280 ≈ 1.0 corresponds to ~0.7 mg/mL for IgG (literature approximation; extinction coefficient is sequence-dependent).

Note: For authoritative, lot-specific properties (concentration, buffer composition, extinction coefficient), consult the CoA/Spec Sheet.

Quality & Grades

This product is supplied as: Carrier Free, ExactAb™, Validated (per Product Data).

• Carrier Free:

  • Meaning: Formulated without protein carriers such as BSA, gelatin, or other stabilizing proteins.
  • Implications: Reduces background in sensitive assays (e.g., mass spectrometry, conjugation chemistries, functional assays where carrier proteins interfere). May be more sensitive to adsorption or freeze–thaw—aliquoting is recommended.

• ExactAb™:

  • Meaning (general): Denotes a recombinant or precisely defined antibody format produced under controlled conditions for sequence and lot-to-lot consistency.
  • Implications: Enhanced reproducibility across experiments; compatible with site-specific conjugation workflows depending on format. Consult CoA/Spec Sheet for clone/sequence/isotype details.

• Validated:

  • Meaning: Internally qualified for defined research applications. Specific tested applications, species reactivity, and validation data are not listed in the Product Data; refer to the CoA/Spec Sheet for details.

What is NOT specified for this item (consult CoA/Spec Sheet):

• Clone ID, isotype/subclass, host species, recombinant expression system, concentration, buffer composition, and any residual preservative content.

Practical notes:

• For conjugation (e.g., HRP, fluorophores), carrier-free format is advantageous. Verify buffer components prior to conjugation to avoid reactive amines or azides if incompatible with your chemistry.

Reaction & Applications

This section (synthetic reaction chemistry) is not typically applicable to antibody reagents.

Relevant applications for this product involve immunoassays and protein detection rather than small-molecule transformations. See:

• Target Specificity: antigen information for ARRDC3.
• Application Protocols: usage guidance in assays such as WB, IHC, IF, ELISA, or IP (if available on the CoA/Spec Sheet).
• Storage & Reconstitution: handling instructions to preserve antibody activity.

Reaction Conditions

Not applicable to chemical synthesis. For experimental conditions in immunoassays, refer to Application Protocols and the CoA/Spec Sheet.

General immunoassay guidance (not item-specific):

• Western blot (WB): Typical primary antibody dilutions range from 1:500 to 1:5,000 depending on antigen abundance and detection method.
• Immunofluorescence (IF)/Immunocytochemistry (ICC): 1:100 to 1:1,000, with fixation/permeabilization optimized per cell type.
• Immunohistochemistry (IHC): Antigen retrieval, blocking, and dilution must be empirically optimized for tissue type and fixation.
• ELISA/IP: Concentration- and matrix-dependent; start with 0.1–2 µg/mL for capture/detection and adjust as needed.

These ranges are general literature guidelines and are not validated for this specific item. Consult the CoA/Spec Sheet for item-specific recommendations.

Safety & Handling

Safety details specific to this item are not provided in the Product Data and should be verified on the SDS.

• GHS classification, signal word, H-statements, pictograms: Not specified for this item; refer to SDS.
• General nature: Aqueous protein reagent intended for research use only; not for human or animal therapeutic or diagnostic use.

Handling guidance (general best practices):

• PPE: Wear lab coat, gloves, and safety glasses to prevent accidental exposure or contamination.
• Aseptic technique: Minimize microbial contamination; keep containers closed when not in use.
• Temperature sensitivity: Avoid repeated freeze–thaw cycles (per Product Data). Aliquot upon first thaw.
• Incompatibilities: Avoid strong oxidizers, proteases, and extreme pH/temperature that denature proteins.
• Spill/cleanup: Wipe with absorbent; decontaminate surfaces with appropriate lab disinfectant. Dispose according to institutional biosafety procedures.
• First aid (general): In case of skin/eye contact, rinse with water. If inhaled or ingested, seek medical attention as per institutional policy. Always consult the SDS for authoritative guidance.

Research use note: For research use only (per Product Data).

Solvent Selection

Not typically applicable. As a protein reagent, the ARRDC3 Antibody is intended for use in aqueous biological buffers (e.g., PBS, TBS) rather than organic solvent systems.

Practical guidance:

• Use isotonic, neutral pH buffers (e.g., PBS pH 7.2–7.4) for dilution and storage unless your assay requires otherwise.
• Maintain low endotoxin and low bioburden conditions. Filter-sterilize buffers (0.22 µm) when preparing working solutions.
• Avoid high percentages of organic solvents that can denature antibodies; if required for specific applications (e.g., certain fixation/permeabilization steps), limit exposure time and validate performance.

Storage & Reconstitution

From Product Data:

• Storage conditions: Store at -20°C. Avoid repeated freezing and thawing.
• Shipping: Ice chest + ice pads (maintain cold chain).

General handling guidance (not item-specific):

• Upon receipt: Inspect for container integrity. Briefly centrifuge to collect contents before first use.
• Aliquoting: Divide into single-use aliquots under sterile conditions to prevent freeze–thaw damage and contamination.
• Short-term storage: For frequent use (days to weeks), 4°C storage can be acceptable if buffer and preservative support stability; otherwise keep at -20°C. Follow CoA/Spec Sheet for item-specific guidance.
• Long-term storage: -20°C or colder as specified. Avoid frost-free freezers with temperature cycling.

Reconstitution/dilution:

• If supplied lyophilized: Reconstitute with sterile, neutral pH buffer (e.g., PBS) to the desired concentration. Allow to dissolve gently at room temperature, then mix by slow inversion; avoid vigorous vortexing or foaming.
• If supplied in liquid form: Dilute only with sterile, compatible buffer immediately prior to use.
• Additives (user-optional, if compatible with your application): 10–50% glycerol for freeze–thaw protection; carrier proteins (e.g., BSA) can reduce adsorption but will negate the carrier-free status and may interfere with conjugation chemistries.

Documentation:

• Record aliquot concentrations, buffer composition, and dates. Always consult the CoA/Spec Sheet for definitive storage and handling instructions specific to SKU Ab089693.

Structure & Identity

ARRDC3 Antibody is an affinity reagent directed against the ARRDC3 (arrestin domain-containing protein 3) target. As an antibody product, small-molecule identifiers (SMILES, InChIKey) do not apply.

• SKU: Ab089693
• Product type: Antibody reagent (research use only)
• Target (antigen): ARRDC3 (Arrestin domain-containing protein 3)

Item-specific identifiers (from Product Data):

• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• CAS/CID/SMILES/InChIKey: Not applicable for antibodies; not specified for this item.

General structural features (literature/general):

• Typical antibody format: IgG-class immunoglobulin (or engineered recombinant equivalent); consists of two heavy and two light chains forming a Y-shaped heterotetramer stabilized by disulfide bonds.
• Approximate composition: Protein biopolymer composed of amino acids with post-translational disulfide linkages; Fc and Fab regions confer effector function and antigen binding, respectively.
• Binding: Variable regions (VH/VL) form the paratope recognizing an epitope on ARRDC3; epitope and clone/isotype are not specified for this item.

2D/architectural description (general):

• Two identical heavy chains (each ~50 kDa) paired with two identical light chains (each ~25 kDa) forming antigen-binding sites at the tips of the Fab arms; Fc domain mediates stability and potential Fc interactions. Exact clone- and isotype-dependent details are not specified for this item.

Synthetic Utility

Not applicable. Antibody reagents are not used as synthetic building blocks or reagents in classical organic synthesis.

Suggested relevant sections:

• Biological Roles: context on the ARRDC3 target.
• Target Specificity and Application Protocols: how the antibody can be used in immunoassays.

Target Specificity

Information available from Product Data:

• Antigen/Target: ARRDC3 (Arrestin domain-containing protein 3)

Not specified for this item; refer to CoA/Spec Sheet for:

• Clone ID and antibody format (monoclonal/polyclonal, recombinant clone)
• Isotype/subclass and light chain type
• Species reactivity (e.g., human, mouse, etc.) and predicted cross-reactivity
• Epitope region (linear/conformational), immunogen details, and affinity/avidity metrics
• Host species and production system
• Validated applications (WB, IHC, IF, ELISA, IP) and recommended dilutions

Note: Product is listed as Carrier Free, ExactAb™, Validated, but specific validation data are not provided in the Product Data above.