Goat Anti-Rabbit IgG H&L (AF405) - Secondary antibody, IgG

Cat. No.: Ab138202

GRADE & PURITY Validated, ExactAb™, High Performance, Ex:401nm, Em:422nm, 1.0 mg/mL

Application

ELISA, Flow Cytometry, IF/ICC

Host species

Goat

Reactive species

Rabbit

Isotype

Goat IgG

Conjugation

AF405

Overview

Goat Anti-Rabbit IgG H&L (AF405), Secondary antibody, could be used for ICC, IF, IHC, Flow, ELISA and so on.
Background：
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 150 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective

Specifications

Product Name

Goat Anti-Rabbit IgG H&L (AF405) - Secondary antibody, IgG

Synonyms

Goat Anti-Rabbit IgG H&L (AF405) | Goat Anti Rabbit IgG H&L (AF405) | Goat Anti-Rabbit IgG H&L-AF405 | Goat Anti Rabbit IgG H&L-AF405 | AF405-Goat Anti-Rabbit IgG H&L | AF405-Goat Anti Rabbit IgG H&L

Specifications & Purity

ExactAb™, High Performance, Validated, Ex:401nm, Em:422nm, 1.0 mg/mL

Host species

Goat

Immunogen

Rabbit IgG

Conjugation

AF405

Excitation (nm)

Violet Laser(405nm)

Emission(nm)

422nm

Ex/Em(nm)

Ex:401nm;Em:422nm

Grade

Em:422nm, Ex:401nm, ExactAb™, High Performance, Validated

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Images

ICC/IF: Goat Anti-Rabbit IgG H&L (AF405) (Ab138202)

Goat Anti-Rabbit IgG H&L (AF405) (Ab138202) - ICC/IF
IF analysis of HeLa cells labeling PABPN1 with purified PABPN1 Rabbit mAb (Ab119979) at 1/200 dilution. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were counterstained with beta Actin Mouse mAb (Ab155822) at 1/500 dilution - Goat Anti-Mouse IgG H&L (AF488) 1/1000. Goat Anti-Rabbit IgG H&L (AF405) (Ab138202) was used as the secondary antibody at 1/500 dilution. Images were taken on the confocal laser scanning microscope.

Application

• Application: Dilution info
• IF/ICC:

  1/100-1/1000 ;

• Flow Cytometry:

  1/500-1/1000 ;