DMEM, High Glucose - Sterile-filtered, BioReagent, Endotoxin tested, for Cell culture, Sterile, with D-glucose (4.5 g/L), phenol red, L-glutamine, HEPES; without sodium pyruvate

Cat. No.: D1372047

GRADE & PURITY Sterile-filtered, BioReagent, Endotoxin tested, for Cell culture, Sterile, with D-glucose (4.5 g/L), phenol red, L-glutamine, HEPES; without sodium pyruvate

Synonyms

DMEM - high glucose | Dulbecco's Modified Eagle Medium

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

Application

Cell Culture

Overview

Dulbecco’s Modified Eagle Medium (DMEM) is a widely used basal medium suitable for the culture of a broad range of mammalian cells, including primary fibroblasts, neurons, glial cells, human umbilical vein endothelial cells (HUVECs), smooth muscle cells, as well as established cell lines such as HeLa, 293, COS-7, and PC12 cells. DMEM was developed based on Minimum Essential Medium (MEM). Compared with MEM, DMEM contains approximately twice the concentration of amino acids and four times the concentration of vitamins, and is additionally supplemented with non-essential amino acids, trace amounts of iron, and sodium pyruvate.

DMEM was originally formulated as a low-glucose medium containing 1,000 mg/L D-glucose. A high-glucose formulation containing 4,500 mg/L D-glucose was subsequently developed and is now widely used for the culture of various cell types. High-glucose DMEM is commonly used for rapidly proliferating cells, cells with relatively low adhesion, myeloma cells used for hybridoma production, cloned cells, transformed cells generated by DNA transfection, primary cells used as viral host cells, single-cell cultures, and vaccine production. For example, it can be used for recombinant erythropoietin (EPO) expression in CHO cells and for hepatitis B vaccine production.

Sodium pyruvate can serve as an alternative carbon source in cell culture. When glucose availability is limited, cells can also metabolize sodium pyruvate. For media formulated without sodium pyruvate, its concentration can be adjusted according to specific experimental requirements, allowing control over the carbon sources available for cellular metabolism. HEPES is an effective biological buffer with low cytotoxicity. Supplementation of culture media with HEPES helps maintain a stable pH over extended periods and effectively reduces the adverse effects of pH fluctuations in the culture medium on cell growth.

This product contains: D-glucose (4,500 mg/L), phenol red, L-glutamine, and HEPES.

This product does not contain: sodium pyruvate.

Specifications

Synonyms

DMEM - high glucose | Dulbecco's Modified Eagle Medium

Specifications & Purity

Sterile-filtered, BioReagent, Endotoxin tested, for Cell culture, Sterile, with D-glucose (4.5 g/L), phenol red, L-glutamine, HEPES; without sodium pyruvate

Stability And Storage

Store at 2-8℃ long term (12 months). Store in the dark.

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, Endotoxin tested, for Cell culture, Sterile, Sterile-filtered

Product Line

Biological Products

Chemical and Physical Properties

• Sensitivity: Light-sensitive