Recombinant proteins frequently accumulate as inclusion bodies in prokaryotic hosts and must be solubilized under denaturing conditions before refolding to regain native structure and activity. Urea and Guanidine Hydrochloride (GdnHCl) are the two workhorse denaturants: urea is cost-effective ...
Topic
Recombinant proteins
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
15 resources · protocols, FAQs & articles
In cell therapy, recombinant protein and antibody drug production, molecular diagnostics, and immunological research, endotoxin (LPS) control is a core requirement for reagent quality. In sensitive cells or in vivo models, contamination as low as
Are recombinant proteins active? Is it possible to do an activity test?
Ubiquitin is a small protein found in all eukaryotes. The covalent attachment of ubiquitin to intracellular proteins is called ubiquitination.
Is it possible to use the vortex shaker to help the lyophilized powder fully dissolve?
Can recombinant proteins of different species be cross-used?
Why didn't I detect the activity of the recombinant protein in the experiment?
What is the role of the protein tag? Does the fusion tag need to be removed during the experiment?
How to operate the reconstitution and preservation of recombinant protein?
How can I save the recombinant protein after re-dissolution?
What are the main differences between the recombinant proteins produced by different expression systems?
Will glycosylation affect the biological activity of recombinant protein?
Why is it necessary to add carrier protein to some recombinant protein solutions?
The TGF-β family (Transforming growth factor-beta family) includes members of TGF-β1, TGF-β2, TGF-β3, BMP (Bone morphogenetic protein), Activin, and Nodal, which play important roles in a variety of biological processes including cell proliferation, differentiation, apoptosis, extracellular ...
Macrophage stimulating protein (MSP) is a protein produced by macrophages. Macrophages play a variety of roles in the immune defense system, including a central role in innate or natural immunity, and were first described as phagocytes by Metchnikoff in the 19th century, and are now known to ...
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Techniques often explored alongside immunological experiments.