PF4V1 Mouse mAb: Protocol & Application Guide

PF4V1 Mouse mAb is a mouse-derived monoclonal antibody targeting Platelet Factor 4 Variant 1 (PF4V1), formulated in PBS and supplied as catalog number Ab351342 for use in standard immunological research applications.

Catalog Number

Ab351342

Antibody Type

Mouse Monoclonal Antibody (mAb)

Target Protein

PF4V1 (Platelet Factor 4 Variant 1)

Formulation

PBS only

Host Species

Mouse

Referenced Source

Absea #KC-46524

Overview of PF4V1 and Antibody Background

PF4V1 (Platelet Factor 4 Variant 1), also known as CXCL4L1, is a member of the CXC chemokine family and is closely related to Platelet Factor 4 (PF4/CXCL4). It is predominantly secreted by activated platelets and is stored in platelet alpha-granules. PF4V1 shares high sequence homology with PF4 but exhibits distinct biological properties, including potent anti-angiogenic and anti-tumorigenic activities. It plays roles in inflammation, hematopoiesis, and immune regulation. The PF4V1 Mouse mAb (Ab351342) is a monoclonal antibody raised in mouse host, providing high specificity for the PF4V1 target, and is suitable for use in multiple immunological assay formats.

Storage and Reconstitution Protocol

Storage Conditions: Upon receipt, store PF4V1 Mouse mAb (Ab351342) at −20 °C for long-term storage. For short-term use (up to 1 month), the antibody may be stored at 4 °C. Avoid repeated freeze-thaw cycles, as these may impair antibody integrity and performance. Aliquot the antibody into single-use volumes before freezing when possible.

Formulation: This antibody is supplied in PBS (Phosphate-Buffered Saline) only, with no carrier protein or preservative stated. Researchers should confirm the exact concentration and any additives by consulting the Certificate of Analysis (CoA) provided with each lot.

Reconstitution: If the product is supplied lyophilized, reconstitute by adding the volume of sterile distilled water or PBS indicated on the vial label. Allow the vial to sit at room temperature for 5–10 minutes and gently mix by swirling. Do not vortex. Centrifuge briefly if particulates are visible before use.

Recommended Application Protocols

Western Blot (WB): Denature protein samples in SDS-PAGE loading buffer, separate by gel electrophoresis, and transfer to PVDF or nitrocellulose membrane. Block with 5% non-fat milk or BSA in TBST for 1 hour at room temperature. Incubate with PF4V1 Mouse mAb at the recommended dilution (refer to CoA) overnight at 4 °C. Wash three times with TBST, then incubate with appropriate HRP-conjugated secondary anti-mouse antibody for 1 hour at room temperature. Detect using a chemiluminescence substrate.

Immunohistochemistry (IHC): Fix and section tissue samples; perform antigen retrieval as appropriate (e.g., heat-induced epitope retrieval in citrate buffer, pH 6.0). Block endogenous peroxidase and non-specific binding. Apply PF4V1 Mouse mAb at recommended dilution and incubate at 4 °C overnight. Use a biotin-streptavidin or polymer-based detection system with HRP and DAB substrate. Counterstain with hematoxylin.

ELISA: Coat 96-well plate with antigen at 1–4 µg/mL overnight at 4 °C. Block with 1% BSA in PBS for 1 hour. Add PF4V1 Mouse mAb at the recommended dilution and incubate for 1–2 hours at 37 °C. Wash and incubate with HRP-conjugated anti-mouse secondary antibody. Develop with TMB substrate and read absorbance at 450 nm.

Note: Optimal dilutions for each application should be determined empirically by the end user. Always refer to the CoA for lot-specific recommendations.

Troubleshooting and Optimization Tips

High Background: Increase blocking time or blocking agent concentration. Increase the number or duration of wash steps. Titrate the primary antibody to a lower concentration.

No Signal: Verify antibody storage conditions and lot expiry. Confirm that the target antigen is expressed in the sample being tested. Check that the secondary antibody is compatible with a mouse primary antibody. Ensure antigen retrieval step (for IHC) was performed correctly.

Non-Specific Bands (WB): Ensure complete transfer and sufficient blocking. Reduce primary antibody concentration. Use a more stringent wash buffer (increase salt or detergent concentration).

Loss of Antibody Activity: Avoid repeated freeze-thaw cycles. Do not leave the antibody at room temperature for extended periods. Confirm that no carrier protein-free formulation has been inadvertently diluted beyond recommended working range.

Quality Control and Safety Considerations

Quality Control: Each lot of PF4V1 Mouse mAb (Ab351342) is referenced to Absea #KC-46524 and should be accompanied by a Certificate of Analysis (CoA) confirming identity, purity, and functional testing data. Researchers are advised to request the CoA for each new lot and to perform lot-to-lot comparison experiments when switching between lots to ensure experimental consistency.

Safety: This product is intended for research use only (RUO) and is not suitable for diagnostic or therapeutic use in humans or animals. Handle according to standard laboratory safety guidelines. The PBS formulation does not contain hazardous preservatives such as sodium azide unless specifically stated on the CoA; however, always consult the Safety Data Sheet (SDS) before use. Dispose of all biological reagents in accordance with local institutional and governmental regulations.

Frequently asked questions

What applications is PF4V1 Mouse mAb (Ab351342) validated for?

PF4V1 Mouse mAb (Ab351342) is generally suitable for standard immunological applications including Western Blot (WB), Immunohistochemistry (IHC), and ELISA. However, validated applications and recommended dilutions are lot-specific; please refer to the Certificate of Analysis (CoA) supplied with your product or contact Aladdin Scientific technical support for confirmation.

How should I store PF4V1 Mouse mAb to maintain its activity?

Store PF4V1 Mouse mAb at −20 °C for long-term preservation. For frequent use within one month, storage at 4 °C is acceptable. To prevent activity loss, aliquot the antibody into single-use volumes before freezing and strictly avoid repeated freeze-thaw cycles.

What is the difference between PF4V1 and PF4, and why does it matter for antibody selection?

PF4V1 (CXCL4L1) and PF4 (CXCL4) are highly homologous chemokines with distinct biological functions; PF4V1 exhibits stronger anti-angiogenic properties compared to PF4. Because of their sequence similarity, cross-reactivity between antibodies targeting each protein is possible. Researchers should use a monoclonal antibody specifically validated against PF4V1, such as Ab351342, to ensure target specificity in their experiments.

Is PF4V1 Mouse mAb suitable for use in flow cytometry?

Flow cytometry suitability for PF4V1 Mouse mAb (Ab351342) is not confirmed in the current product documentation. Researchers interested in this application should contact Aladdin Scientific technical support or perform in-house validation experiments to determine feasibility and optimal conditions.

Can I use PF4V1 Mouse mAb for diagnostic purposes?

No. PF4V1 Mouse mAb (Ab351342) is strictly for research use only (RUO) and has not been approved or validated for clinical diagnostic, therapeutic, or veterinary diagnostic applications. Use in such contexts would be outside the intended and validated scope of this product.

References

1. Struyf S, Burdach S, Proost P, et al. The CXC chemokine platelet factor 4 variant 1 (CXCL4L1/PF4alt) inhibits melanoma and lung carcinoma growth and metastasis by preventing angiogenesis. Cancer Letters. Review-level textbook reference — specific details need verification.
2. Vandercappellen J, Van Damme J, Struyf S. The role of CXC chemokines and their receptors in cancer. Cancer Treatment Reviews. Textbook-level reference — specific volume, page and author details need verification.
3. Absea Biotechnology. Product Data Sheet: KC-46524. Internal reference cited in Aladdin Scientific product documentation for Ab351342.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.