Experimental methods of Agrobacterium-mediated transformation of barley
Agrobacterium-mediated transformation of barley has been widely utilized over the last decade and it offers advantages over gene gun-mediated transgenic techniq
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Fluorescence in situ hybridization for the detection of transgene fragments integrated in plant genomes experiments
Over the past 30 years, fluorescent chromosome analysis techniques have facilitated the understanding of genome construction and deepened knowledge of the organ
Gene insertion site and pattern experiments
The study of insertion patterns and loci of exogenous genes in transgenic plants greatly contributes to the understanding of the mechanisms of integration, expr
Substantial equivalence (transcriptomics) experiments
In Western Europe, for transgenic crops to be approved for commercial production, proof of their substantial equivalence to conventionally bred crops needs to b
Experiments on the design and management of field trials of transgenic crops
With the development of transgenic technology systems, the transfer of heterologous genes into the plant genome has become possible, which provides a new avenue
Substantial equivalence (proteomics) experiments
Wheat, as one of the major global crops, is processed into a range of food products for consumer consumption. Therefore, it is of great importance to determine
Plant mitochondria preparation and its substructure hierarchical separation experiments
Plant cell mitochondria perform a number of important biochemical processes. The most important of these is the oxidative degradation of organic acids via the t
Xylem and phloem sap protein extraction experiments
It is well known that the xylem and primordial vasculature in higher plants are responsible for the long-distance transport of small nutrient molecules. However
Plant whole protein extraction by trichloroacetic acid-acetone method
In this chapter we describe an efficient method suitable for whole protein extraction from various plant tissues. The method utilizes trichloroacetic acid and a