Experiments on bidirectional electrophoresis techniques in plant proteomics
Bidirectional gel electrophoresis and mass spectrometry for protein identification using fixed pH gradients (IPGs ) are currently the main techniques for proteo
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Experiments on the quantitative analysis of two-dimensional gels
Two-dimensional electrophoresis, as an important experimental tool for comparative proteomics studies, has been applied to conveniently reflect the differences
Experiments on the solubilization of proteins by descaling and leaving agents prior to bidirectional electrophoresis
Because of its outstanding ability to separate total proteins, bidirectional gel electrophoresis can be applied to the separation of all types of proteins. Howe
Protein identification using nanoscale liquid chromatography-tandem mass spectrometry
Tandem mass spectrometry is an effective technique for peptide characterization based on the fragmentation pattern of the peptide, also known as MS/MS scanning.
Plant proteomics and glycosylation experiments
Like other eukaryotic cells, glycosylation is one of the most studied post-translational events in plant cells. Depending on the type of connection between the
Isolation and characterization of membrane proteins and their functional analysis-GFC/IEC/SDS-PAGE and MALDI-TOF-MS experiments
Prior to the identification of membrane proteins by matrix-assisted laser-resolved time-of-flight mass spectrometry (MALDI-TOF-MS), these proteins need to be se
2-DE PROTICdb database experiment for proteomics
PROTICdb is a web-based database designed primarily to store and analyze plant proteomic data obtained by 2D polyacrylamide electrophoresis and mass spectrometr
Protein Edman sequencing experiments on 2D gels
Immunoblotting using polyvinylidene difluoride (PVDF ) membranes is the most commonly used method for Edman sequencing. Protein samples are transferred from a 2
Peptide mass fingerprinting and protein identification by MALDI-TOF method
MALDI-TOF peptide mass fingerprinting (PMF) is a faster and more convenient method for identifying proteins. The genomes studied have been sequenced and annotat