Isolation of cytoplasmic RNA from mammalian cells
The following method is a simple and effective method for purifying cytoplasmic RNA from tissue culture cells, which appeared at an earlier stage of RNA isolati
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Whole blood RNA extraction
Whole blood RNA extraction can be used (1) to study the mechanism of RNA interference, etc.; (2) as a template for reverse transcription; and (3) for other stud
cDNA synthesis technology
After the mRNA of the target gene has been identified by in vitro cell-free translation, the first strand of cDNA can be synthesized in the steps described in t
Construction of siRNA expression vectors
The construction of siRNA expression vectors can be applied to: (1) as a powerful tool for gene function analysis in the post-genomic era; (2) genomics and cell
Viral RNA Extraction
Viral RNA extraction can be (1) used for the study of RNA viral replication mechanisms, (2) used for reverse genetics studies, and (3) used for other studies in
Northern blotting and narrow line hybridization analysis of RNA
Northern blot is one of the most rigorous methods to study gene expression, which can quantitatively analyze the expression abundance of a specific mRNA in tiss
Principles, materials and procedures for denaturing agarose gel electrophoresis of RNA
This experiment is mainly used to determine the integrity of RNA extracts.
RNA extraction from formalin-fixed, paraffin-embedded tissues
Formalin-fixed, paraffin-embedded tissues from the pathology department can be used for RNA expression analysis. RNA isolated from biopsy, surgical, and cadaver
Rapid isolation of total RNA from mammalian cells
SDS lyses the cells and extracts the total RNA by acid phenol graded extraction, which is a suitable method for treating cells with relatively low mRNA content