Extraction of RNA from tissues by the CsCl method
Because some tissues (e.g., pancreas and uterus) contain high concentrations of endogenous RNAases, more care must be taken in purifying tissue-derived RNA.
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RNA extraction from formalin-fixed, paraffin-embedded tissues
Formalin-fixed, paraffin-embedded tissues from the pathology department can be used for RNA expression analysis. RNA isolated from biopsy, surgical, and cadaver
Extraction and isolation of rat liver rRNAs
Rat liver was homogenized, the supernatant was centrifuged, and RNA was extracted with a mixture of phenol and m-cresol. rRNA and mRNA were separated in the pre
Effect of different methods of treating tissues on the yield of extracted RNA
RNA Laboratory Techniques Manual (Molecular Cloning - Laboratory Guide Series)
Extraction of RNA from cultured cells by the CsCl method
RNA preparation by guanidine thiocyanate extraction and cesium chloride centrifugation is suitable for isolation of RNA from cells with difficult separation of
Rapid isolation of total RNA from mammalian cells
SDS lyses the cells and extracts the total RNA by acid phenol graded extraction, which is a suitable method for treating cells with relatively low mRNA content
nuclear transcription analysis
Nuclear runoff (runoff) analysis can be used to examine the transcriptional efficiency of specific genes in isolated cell nuclei. For runoff analysis, the nucle
Ligation of RNA molecules with T4 RNA ligase
T4RNA ligases have been used to generate many site-specific modified RNAs, especially oligonucleotide-modified and tRNA-modified with antisense codon tRNAs.In a
RNA radiolabeling
Intramolecular labeling of RNA during transcription, post-transcriptional labeling of the 5' end of RNA using T4 polynucleotide kinase and labeling of the 3' en