Isolation of mouse macrophages
Introduction of a method for the isolation of mouse macrophages by J.E. Colligan et al, translated by Xuitao Cao et al. This experiment is from the "Compendium
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Phenotypic assay of mouse macrophages
Due to the lack of characteristic surface markers of macrophages or macrophages at different stages of differentiation, macrophages are currently identified bas
Detection of α-, β- and γ-interferon-induced antiviral activity
The cells used in the assay are mouse L 929 fibroblasts, which have a high sensitivity to interferon and V S V . The cells should be derived from laboratories t
Isolation and expansion of human NK T cells
This protocol allows the identification and quantitative analysis of trace amounts of N K T cells. Effective measures should be taken to block non-specific stai
Presentation of exogenous antigens to T cells
For granular antigens or liposome-encapsulated antigens, macrophages were applied as antigen-presenting cells. Author: J.E. Colligan et al, Translator: Xuitao C
Detection of human lymphocytes in whole blood by flow cytometry
This method analyzes whole blood cells compared to analyzing Ficoll-isolated single nucleated cells, thus taking less time, requiring a smaller sample size, and
In vivo induction and detection of antibodies
Detection of Antibody Production in Vivo by ELISA by J.E. Colligan et al, Translated by Xuitao Cao et al. This experiment is from the "Compendium of Immunology
ELISA for detection of antibodies
This method detects IgG, I g M and IgA in serum or cell culture supernatants (Fig. 8. 1 0 . 1 ). The color of the liquid in the assay wells is directly proporti
B Detection of cell-secreted antibodies
The following is an example of mitogen-induced antibody production by B cells using American continental protein. This method has been adapted for use with a va