Preparation of EBV-transformed B cells
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Experimental effects of anticancer drugs on ⅡDNA topoisomerase
The effect of anticancer drugs on DNA topoisomerase activity assay was performed using agarose gel electrophoresis to determine the effect of anticancer drugs o
Isolation of B-cell subpopulations by immunomagnetic bead assay
Anti-CD 19 labeled B cells, which have relatively low signal intensity, show mitogenic and antibody secretory responses similar to those obtained by negative sc
Detection of NK/LAK cell killing activity
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
Isolation and purification of IgG
IgG isolation and purification experiments can be used to: prepare enzyme labeled antibodies or fluorescent antibodies.
Cultured dendritic cells from mouse bone marrow precursor cells
Dendritic cells (DCs) are very few in number in vivo (less than 1% of splenic adherent cells), and the time-consuming isolation of DCs from vivo and their low c
Macrophage chemotaxis assay
Macrophage migration is the primary condition for its immune activity at the site of infection and injury, and macrophage chemotaxis reflects its migration abil
B Identification of functional subpopulations of cells
Peripheral mature B cells can be divided into several subpopulations. Traditionally, B cells have been divided into two subpopulations, CD5+ B1 cells and CD5 B2
Isolation and purification of mouse peritoneal macrophages
Macrophages (MΦ) are mature cells formed by the migration of blood monocytes into tissues, and are widely distributed in the spleen, liver, abdominal cavity, n