Melanocyte culture
After trypsin digestion, epidermal slices isolated from skin slices were dispersed with EDTA and then incubated with serum-free culture medium supplemented with
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organ culture experiment
Since the desired organ culture technique involves placing the tissue in a position ideal for gas and nutrient exchange, most of these techniques involve placin
Separation of cell colonies by radiation
Place the culture bottles upside down under an X-ray machine or 60Co source and shade the desired colonies with a lead block.
Microscope digital photography
Open the microscope and calibrate the optical path. Focus on the relevant area of the specimen. Check the connection between the camera and the computer, direct
Preparation and Sterilization of D-PBSA
The dry powder was dissolved by constant stirring, adjusted to final volume, tested for pH and conductivity, and autoclaved in predetermined portions.
Magnetic Activated Cell Sorting (MACS)
A buffy coat or another mixed cell suspension is mixed with antibody-conjugated microbeads, diluted and placed in a magnetic separation column. Cells bound to t
Preparation and sterilization of glassware
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
DNA assay with Hoechst 33258
Homogenize cells or tissues in buffer, followed by sonication. An amount of the cell suspension is mixed with Hoechst 33258 and the fluorescence is determined.
Crystalline violet staining
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)