Chromosome Preparation
Cells blocked in mid-division were fixed, swollen in hypotonic solution, dropped on slides, stained, and observed [ Rothfels and Siminovitch, 1958; Rooney and C
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Giemsa stain
Cultures were fixed with methanol and stained directly with undiluted Giemsa's solution, then the stain was diluted 1:10, the cultures were washed, and observed
Cell counting experiments with blood cell counting plates
Digest the monolayer culture with trypsin, or take a sample from the suspension culture; prepare a coverslip and add the cells to the small chamber of the hemoc
Methotrexate resistance and DHFR amplification
The cells are sequentially placed in a series of concentrations of MTX for several weeks, with periodic changes to fresh medium containing the same drug concent
Use of the inverted microscope
Place the culture on the microscope's stage, turn on the power, select the appropriate lens and focus on the specimen. Adjust the condenser and phase contrast r
Experiments for plotting growth curves of cells cultured in monolayers in culture flasks
Following passaging culture, cells assume growth forms characterized by delayed, exponential or logarithmic, and stationary (plateau) phases. The logarithmic an
Experiments for drawing growth curves of cells cultured in monolayers in multiwell culture plates
Three sets of cells were cultured in multiwell plates at three different cell concentrations, and cells in one plate were counted each day before reaching plate
Preparation of media with dry powder
Dissolve all the dry powder in the package with appropriate amount of ultrapure water: put the container on the magnetic stirrer and add the dry powder while st
Preparation of special media
Prepare storage concentrates and freeze for preservation. Thaw, mix, aseptically filter and store when needed.