DNA contamination removal assay after elution
The following protocol is a modification of the Dilworth and Huang protocol (DilworthandMcCarey1992;Huangetal.2000). It can be used to remove DNA contamination
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HE staining of slices for LCM
Prior to LCM, cut tissue samples are fixed, stained, and dehydrated if necessary. Authors use classical HE staining for visceral and lymph node tissues. Once th
Single-stranded cDNA synthesis
This protocol utilizes total RNA because if 18SrRNA is used as an internal control, RNA with poly(A) cannot be used in subsequent experiments. and the RNA must
Real-time PCR experiment
This method utilizes the PlatinumQuantitativePCRSuperMix-UDG. When performing real-time PCR, there should be a set of equipment that can detect the fluorescence
Parameter experiments for real-time PCR
LUX primers are labeled with FAM (6-carboxyfluorescein) or JOE (6-carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein). In reactions without a specific template,
Establishment of a real-time PCR quantitative analysis experiment
The process of establishing a quantitative real-time PCR assay for a new target molecule involves: (i) a design step for the selection of PCR primers and probes
Experiments for the preparation of ablated cDNA or genomic DNA libraries
A total of 2ug of genomic DNA or cDNA is required for a single bi-directional ablation.Most methods for isolating RNA and genomic DNA are suitable for this expe
Preparation of template DNA, organization of experiments and PCR amplification experiments
Genomic DNA can be isolated by a variety of methods, depending primarily on the type (blood or tissue) and amount of initial material. All prepared DNA should b
Reverse transcriptase in situ PCR assay
This section describes an abbreviated method for in situ PCR, specifically reverse transcriptase in situ PCR. Each of these experimental procedures is discussed