Magnetic particle-based purification of PCR products
MagneSil is a paramagnetic silica particle that acts as a mobile solid-phase material for capturing double-stranded DNA fragments. The target complexes adhering
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Purification of PCR fragments from agarose gels
The Wizard SV Gel and PCR Purification System is a method for the direct purification of PCR fragments from agarose gels or from PCR amplification reaction mixt
RNA purification experiments with Trizol
The production of phenol-based reagents for RNA isolation is based on Chomczynski protocols. These protocols are most useful when obtaining RNA from RNAase-rich
Clean purification experiments for stained ends
Automated fluorescence sequencing using ABIPRISMRigDye end chemistry is one of the methods for efficient DN eight sequence determination. Sequence gradients are
RNA preparation from paraffin-embedded fixed tissues
This protocol was published by Godfrey et al. as an improvement on Fisher's protocol. Previously, protocols for purifying RNA from fixed tissues have been publi
Silica membrane-based purification of PCR products
The Wizard SV96 PCR Purification System provides a membrane-based method for high-yield PCR purification from 96-well plates. This experiment was derived from P
An experimental method for the isolation of genomic DNA based on magnetic beads
The Wizard Magne Sil Genomic DNA Purification System uses a solid-phase paramagnetic silica pellet to purify genomic DNA, replacing vacuum filtration and centri
Genomic DNA isolation experiment based on silica membrane
Silica chemistry has become one of the methods for purifying high-quality genomic DNA from samples such as mouse tail clippings, animal tissues, and tissue cult
RNA purification experiments from plant tissues using ConcertPlant reagents
Outlined below is the protocol that comes with ConcertPlant Reagent (Invitrogen). The protocol is not phenol-based, but requires the addition of chloroform. Thi