Experiments on lysis of cultured cells by depressurization of nitrogen compartments
Source: Laboratory Guide to Proteins and Proteomics
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Lysis experiments of cultured animal cells, yeast and bacteria for immunoblotting
Source: Laboratory Guide to Proteins and Proteomics
Total protein extraction from adipose tissue and cells
Extraction of total protein from adipose tissue and cells
Fluorescent staining of frozen sections for amyloid (AMYLOID) thioflavine T
Fluorescent staining of protein fibers
Far Western analysis of protein interaction experiments
When analyzing protein interactions using Far Western blot, the target protein is immobilized on a solid support membrane and then probed with a non-antibody pr
Recombinant MVA in vivo immunostaining assay
In vivo immunostaining can be used for the detection of modified poxvirus Ankara (MVA) and also for the identification of proteins expressed on the cell surface
Affinity purification assay of GST fusion proteins
This experiment describes the application of proteins fused to glutathione-S-transferase (GST fusion proteins) to affinity purify other proteins, also known as
Dialysis for the removal of sodium N-dodecyl sarcosinate
This experiment describes how much time it takes to remove sodium N- dodecyl sarcosinate by dialysis. This experiment is from the Laboratory Guide for Protein P
Formaldehyde denaturing gel electrophoresis to identify RNA experiment
The purpose of this experiment is to learn how to identify RNA by formaldehyde denaturing gel electrophoresis. Agarose gel electrophoresis is often used for the