Carrier amphoteric electrolyte pH gradient isoelectric focusing experiment
Isoelectrofocusing, abbreviated as IEF or EF, was established in the 1960s as a means of separating and analyzing proteins, and has evolved rapidly over the pas
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UV spectra of pure proteins A280 nm/A260 nm Experimental
A relatively simple and effective measure of protein purity is obtained by determining the ultraviolet (UV) spectra of purified proteins. Even if sodium dodecyl
Determination of sucrase activity at various levels and calculation of purification rate experiments
The rate of sucrose hydrolysis was determined by measuring the amount of reducing sugars (glucose and fructose) produced, and in this experiment, the unit of su
Extraction of total plant RNA
Grind the plant material in liquid nitrogen, make some cells broken, further make the plant cells lysed in lysis solution, precipitate protein with sodium aceta
Tm value measurement of DNA
When the dilute salt solution of DNA is heated to 80~100 ℃, the double helix structure will be disintegrated, the two strands will be separated, and a random c
Dissolution, refolding and ion exchange chromatography experiments on inclusion body precipitates (σ32)
This experiment describes the solubilization, refolding and ion exchange chromatography of inclusion body precipitation (σ32). This experiment was derived
DEAE cellulose column chromatography purification of enzyme protein experiment
The aim of this experiment was to further purify sucrase protein by column chromatography, using DEAE cellulose resin as an ion-exchange agent for hierarchical
Measurement of extinction coefficients (Scopes method)
Scopes published a simple method for determining the extinction coefficient (E) of proteins (Scopes, 1974). This method is based on the fact that although most
Protein kinase C isoform analysis experiment
Protein kinases are analyzed using labeled donor substrates, and the accumulation of markers in protein or peptide acceptor substrates is readily detected when