Determination of key enzymes and important products in the anaerobic oxidation pathway
Glucose undergoes a 10-step metabolic reaction of glycolysis to produce keto acid, which is then further produced as lactate or decarboxylated into acetyl coenz
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3228 articles
Preparation of DNAase I Footprint Probes
DNA enzyme I footprinting analysis is an effective method for detecting and characterizing the sequence-specific binding ability of transcription factors to DNA
Determination of total protein content based on protein elemental composition
Determination of total protein content based on the elemental composition of proteins is a method of direct analysis based on the characteristics of the element
Determination of NADPH-cytochrome C (P-450) activity
NADPH-Cytochrome C (P-450) is a heme protein that is a terminal oxidase due to the fact that when it is in its reduced form, it combines with carbon monoxide to
Pentose phosphate pathway
The pentose phosphate pathway is an oxidative catabolism of glucose that produces NADPH and synthesizes ribose phosphate, which provides the raw material for nu
GST pull-down analysis
Protein-protein interactions play an important role in a variety of life processes such as biocatalysis, transporter, signaling, immunity, cellular regulation,
Experiments for precise molecular weight determination of proteins
The exact molecular weight of a protein is the sum of all the atomic weights contained in the molecule. Currently, there is one main method used to determine th
CUT&RUN-seq protocol
Targets and Release Using Nuclease (CUT&RUN) experimental technology is an emerging chromatin analysis method for studying protein-DNA interactions. It uses antibodies to specifically label target proteins, and then uses nucleases bound to antibodies (such as pAG-MNase) to cut DNA fragments that ...
Acid phosphatase activity assay
Acid phosphatase (ACP) is widely found in various cells, and is often abnormally expressed in some cancer cells. Sodium p-nitrophenol phosphate is used as the substrate of ACP, which can be hydrolyzed under alkaline conditions to produce yellow p-nitrophenol, and ACP can be quantitatively ...