Identification of IgG purity by polyacrylamide gel electrophoresis
Since the concentration of the polyacrylamide gel can be prepared on demand, two electrophoresis systems, the "Continuous System" and the "Discontinuous System,
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Experimental determination of protein concentration by bicinchoninic acid (BCA) assay
The bicinchoninic acid (BCA) assay is a new and improved Lowery assay that has recently been developed, with a simple reaction and virtually no influence of int
Separation of lactate dehydrogenase isozymes by agarose gel electrophoresis assay
Lactate dehydrogenase is present in all glycolytic cells, and catalyzes the dehydrogenation of lactate to form pyruvate or the reduction of pyruvate to lactate
TNF-α Biological Activity Assay
One of the biological activities of TNF is its ability to directly kill tumor cells.TNF binds to the corresponding receptor and then moves into the cell, where
Dialyzed to obtain soluble refolding σ32 Monomer experiments
This experiment describes what dialysis conditions lead to the highest yield of soluble refolded σ32 monomer. This experiment is from the guide to protein
Experiments for the determination of the isoelectric point of proteins
The isoelectric point of a protein is one of the important properties of proteins. Determination of isoelectric point of proteins is important for understanding
X-ray crystallography
The ability of protein molecules to crystallize has been reported for more than 170 years, and the discovery of X-rays by Rntgen WC in 1895 and X-ray diffractio
Determination of total protein content based on protein light absorption properties
Determination of total protein content based on protein light absorption properties is based on the light absorption properties of different chemical bonds of p
Experiment to determine the content of cell membrane proteins
Membrane proteins are protein molecules present in the cytoplasmic membrane of cells and play important roles in intracellular molecular transport, energy metab