Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
2-Mercaptoethylamine•HCl (2-MEA) is a mild reducing agent suitable for selectively cleaving hinge-region disulfide bonds of IgG heavy chains. Several popular methods for conjugation or immobilization of antibodies involve covalent attachment specifically through sulfhydryl (−SH) groups.
If I need to label an antibody with SMCC-activated R-phycoerythrinand then use the antibody coupled with phycoerythrin as an ELISA, what other reagents are needed? Is there a specific procedure?
Updated Sep 202412 min read✓ Reviewed
Related topics
Techniques often explored alongside immunological experiments.
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