Step-by-step protocol for Plant Genomic DNA Extraction Kit (CTAB Crude Extraction Method, P1527546). Learn principle, procedure, and troubleshooting tips.
Topic
DNA extraction
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
12 resources · protocols, FAQs & articles
Extract high-purity λ phage genomic DNA with Aladdin's PEG precipitation kit (P1527075). Reliable protocol for downstream cloning, sequencing, and restriction analysis.
Explore IPA's properties, purity grades, and lab applications—sterilization, DNA extraction, and cleaning—plus safety and storage tips from Aladdin Scientific.
Explore the physicochemical properties, laboratory applications, and safe handling of 3-Methyl-1-butanol (isoamyl alcohol) from Aladdin Scientific.
Learn about TEN Buffer (1×, pH 8.0) composition, preparation, applications in DNA/RNA work, and best practices for nucleic acid research labs.
Learn about TEN Buffer (1×, pH 7.2): composition, preparation, applications in nucleic acid work, and storage. Expert answers for research scientists.
Learn how the Endo-free Plasmid Mini Kit (D1088768) delivers high-purity, endotoxin-free plasmid DNA for transfection and sensitive downstream applications.
Explore Zymolyase from Arthrobacter luteus: mechanism, yeast spheroplast preparation, DNA/RNA extraction applications, and selection guidance for research labs.
Phenol-based nucleic acid extraction separates DNA or RNA from proteins, lipids, and cellular debris through sample lysis, protein denaturation, and aqueous-organic phase partitioning. Neutral or mildly alkaline phenol systems are generally used for DNA and total nucleic acid extraction, whereas ...
The core goal of DNA extraction is to obtain intact, pure DNA suitable for downstream experiments from cells, tissues, blood, plants, microorganisms, or environmental samples. Method selection should consider sample type, DNA fragment integrity, inhibitor removal capacity, throughput ...
In plant nucleic acid extraction, the main challenge usually lies not in lysis itself, but in how effectively nucleic acids can be separated from polysaccharides, polyphenols, pigments, proteins, lipids, and residual cell wall components after lysis.
GITC is a strong denaturant and nuclease inhibitor. It is commonly used in the form of high-concentration solutions for life science research such as nucleic acid extraction, sample preservation, virus inactivation, protein denaturation, and pre-processing of molecular diagnostics.
Related topics
Techniques often explored alongside immunological experiments.