ADAR1 is an important RNA-editing enzyme that catalyzes adenosine-to-inosine (A-to-I) editing in double-stranded RNA (dsRNA), reducing the recognition of endogenous dsRNA by nucleic acid-sensing systems such as MDA5, PKR, and ZBP1. Tumor cells can exploit ADAR1 to maintain RNA immune tolerance, ...
Topic
Double-stranded RNA
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
2 resources · protocols, FAQs & articles
Experimental Judgment in mRNA In Vitro Transcription: Template Design, 5′ Capping, and dsRNA Control
The outcome of mRNA in vitro transcription does not usually depend only on whether an RNA of the target length has been obtained. Whether the template elements are arranged appropriately, whether the 5′ cap structure matches the intended downstream use, and whether key impurities such as ...
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Techniques often explored alongside immunological experiments.