Uric acid is the major end product of purine catabolism in humans and exists primarily as free uric acid or urate in blood, urine, and tissues. Uric acid quantification is a commonly used technique in clinical biochemical analysis and metabolic research and is widely applied in hyperuricemia ...
Topic
High-performance liquid chromatography
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
5 resources · protocols, FAQs & articles
In high-performance liquid chromatography (High Performance Liquid Chromatography, HPLC), octadecylsilane-bonded stationary phase (Octadecylsilane, C18) is the most commonly used reversed-phase stationary phase. The main separation basis of C18 is hydrophobic partitioning: the more hydrophobic a ...
Amino acid analysis is a fundamental technical component in biological sample analysis, nutritional evaluation, metabolism research, and fermentation process control. Its analytical targets include free amino acids, total amino acids after hydrolysis, specific single amino acids, and certain ...
Hydroxyproline determination is a classical quantitative technique in collagen-related research and is widely used in fibrosis assessment, tissue-remodeling studies, collagen-material analysis, and pharmacodynamic evaluation.
Vitamin C content determination is not merely a matter of quantification, but rather a comprehensive analytical issue involving definition of the target analyte form, sample stabilization procedures, methodological selection, and the interpretive boundaries of analytical results. Because vitamin ...
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Techniques often explored alongside immunological experiments.