Coelenterazine (Coelenterazine, CTZ, CAS 55779-48-1) is a representative marine luciferin that is widely used as the substrate and light-emitting chromophore for multiple marine luciferases and the jellyfish photoprotein (Aequorin). By tuning spectral position, emission intensity, reaction ...
Topic
Luciferase
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
6 resources · protocols, FAQs & articles
In firefly luciferase reporter systems, signal quality is jointly determined by substrate accessibility, effective substrate concentration, reaction kinetics, and optical acquisition parameters. Substrate identity alone is rarely the only determinant; without control systems and parameter ...
Luciferase, as a core tool for bioluminescence detection, has been used for decades yet continues to inspire new methods and applications.Recent studies from both biomolecular engineering and optical imaging engineering point toward a common goal: to simultaneously and distinguishably observe ...
General consideration when shopping or using luciferin/luciferase
A Helpful Introduction to the ATP Bioluminescence Assay
A Course on Luciferase Assays
Related topics
Techniques often explored alongside immunological experiments.