Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Polyclonal, monoclonal, and recombinant antibodies are all suitable for western blotting. Polyclonal antibodies are a collection of many monoclonal antibodies that can vary between immunizations and batches. Polyclonal antibodies recognize multiple epitopes of an antigen and are therefore ...
In-cell ELISA (also known as cell-based ELISA, cell western blot, or cytoblot) is a widely accepted immunocytochemistry assay that uses a colorimetric or fluorescent readout to quantify a target protein or post-translational modifications of a target protein in cultured cells.
Updated Jun 2025·33 min read
Related topics
Techniques often explored alongside immunological experiments.
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