MAPK11 Antibody - Primary antibody, specific to MAPK11, Rabbit IgG

Cat. No.: Ab119724

GRADE & PURITY Carrier Free, Validated, ExactAb™, 0.6 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human MAPK11 Antibody, Polyclonal antibody, could be used for WB, IHC and so on.
Application
WB: 1/500 - 1/1000
IHC: 1/50 - 1/100
Protein Function
Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment, by cytokines, or by environmental stress. Phosphorylates preferentially transcription factor ATF2.
Post-translational
Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.

Specifications

Product Name

MAPK11 Antibody - Primary antibody, specific to MAPK11, Rabbit IgG

Synonyms

Human p38Beta MAP kinase mRNA complete cds antibody | MAP kinase 11 antibody | MAP kinase p38 beta antibody | MAPK 11 antibody | Mapk11 antibody | Mitogen activated protein kinase 11 antibody | Mitogen activated protein kinase p38 2 antibody | Mitogen act

Specifications & Purity

ExactAb™, Validated, Carrier Free, 0.6 mg/mL

Host species

Rabbit

Specificity

MAPK11

Immunogen

A synthetic peptide derived from human MAPK11. (AA 251-364).

Positive Control

WB: HeLa, MCF7 and Jurkat cell lysates. IHC: Human brain tissue. IF/ICC: HeLa cells

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Concentration

0.6 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: MAPK11 Antibody (Ab119724)

MAPK11 Antibody (Ab119724) - Western Blot
All lanes: MAPK11 Antibody (Ab119724) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 41 kDa
Observed band size: 38 kDa
Exposure time: 60.0 s

Western Blot: MAPK11 Antibody (Ab119724)

MAPK11 Antibody (Ab119724) - Western Blot
All lanes: MAPK11 Antibody (Ab119724) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 42 kDa
Observed band size: 39 kDa
Exposure time: 100.0 s

IHC: MAPK11 Antibody (Ab119724)

MAPK11 Antibody (Ab119724) - IHC
Immunohistochemistry analysis of paraffin-embedded Human brain using MAPK11 Antibody (Ab119724). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: MAPK11 Antibody (Ab119724)

MAPK11 Antibody (Ab119724) - IF/ICC

IF analysis of MAPK11 Antibody (Ab119724) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with MAPK11 Antibody (Ab119724) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Application

• Application: Dilution info
• WB:

  1/500-1/1000

• IHC:

  1/50 - 1/100;

• IF/ICC:

  1/200;

Associated Targets(Human)

MAPK11 Tchem Mitogen-activated protein kinase 11 (0 Activities)

Discover Target: MAPK11

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

MAPK11 Tchem MAP kinase p38 beta (2785 Activities)

Discover Target: MAPK11

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action

References

1. Peng Zhu, Jianqiang Qian, Zhongyuan Xu, Chi Meng, Ji Liu, Wenpei Shan, Weizhong Zhu, Yongjun Wang, Yumin Yang, Wei Zhang, Yanan Zhang, Yong Ling.  (2020)  Piperlonguminine and Piperine Analogues as TrxR Inhibitors that Promote ROS and Autophagy and Regulate p38 and Akt/mTOR Signaling.  JOURNAL OF NATURAL PRODUCTS,      [PMID:33026807] [10.1021/acs.jnatprod.0c00599]