Phosphate acetyltransferase (PTA） , CAS No.9029-91-8

CAS: 9029-91-8 Cat. No.: P1431341 EC Number: 232-855-2

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥3000 U/mL; ~3500 U/mg protein

Accession #

P39646

Bioactivity

≥3000 U/mL; ~3500 U/mg protein

Overview

High purity recombinant phosphotransacetylase (Bacillus subtilis) for use in research, biochemical enzyme assays and analytical testing applications. 

Specificity: 

Catalyses the reaction: Coenzyme A + acetyl phosphate = acetyl-CoA + phosphate

Temperature Optima: 25°C

pH Optima: 7.4

Application examples: Applications for the measurement of acetic acid in the food, fermentation, wine, beverage and dairy industries.

PROPERTIES 

1. ELECTROPHORETIC PURITY: 

- Single band on SDS-gel electrophoresis (MW ~ 36,953) 

- Single major band on isoelectric focusing (pI ~ 5.3) 

2. PHYSICOCHEMICAL PROPERTIES: Recommended conditions of use are at pH 7.4 and up to 25°C. 

3. STORAGE AND USE CONDITIONS/RECOMMENDATIONS: The enzyme is supplied as an ammonium sulphate suspension containing 0.5 mg/mL BSA and should be stored at 4°C. For assay, this enzyme should be diluted in 100 mM Tris.HCl buffer, pH 7.4 containing 1 mg/mL BSA. Swirl to mix the enzyme suspension immediately prior to use. 

Specifications

Product Name

Phosphate acetyltransferase (PTA） , CAS No.9029-91-8

Synonyms

Acetyl-CoA:orthophosphate acetyltransferase | phosphate acetyltransferase | phosphoacylase | phosphotransacetylase | PTA

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,≥3000 U/mL; ~3500 U/mg protein

Bioactivity

≥3000 U/mL; ~3500 U/mg protein

Accession #

P39646

Predicted molecular weight

36,953

CAS

9029-91-8

Enzyme Commission Number

EC 2.3.1.8

Molecule Type

Enzyme

Product Line

Biological Products

Storage and Shipping

Concentration

≥3000 U/mL; ~3500 U/mg protein

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ long term. Upon receipt, it is recommended to aliquot.

Unit definition

One Unit of phosphotransacetylase activity is defined as the amount of enzyme required to release one µmole of acetyl-CoA per minute from Coenzyme A (0.44 mM) in Tris.HCl buffer (86 mM) pH 7.4 at 25°C.