PITPNB Mouse mAb - Primary antibody, specific to PITPNB

Cat. No.: Ab121957

GRADE & PURITY KO Validation, ExactAb™, High Performance, See COA

Application

IHC, WB

Host species

Mouse

Reactive species

Human, Mouse, Rat

Isotype

Mouse IgG1

Clone Number

OTI5D11

Conjugation

Unconjugated

Overview

Mouse anti Human PITPNB Antibody, Monoclonal (OTI5D11), could be used for WB, IHC and so on.

Specifications

Product Name

PITPNB Mouse mAb - Primary antibody, specific to PITPNB

Synonyms

OTTHUMP00000198846 antibody | OTTHUMP00000198847 antibody | Phosphatidylinositol transfer protein beta isoform antibody | Phosphatidylinositol transfer protein, betaantibody | PI TP beta antibody | PI-TP-beta antibody | PIPNB_HUMAN antibody | Pitpnb antib

Specifications & Purity

KO Validation, ExactAb™, High Performance, See COA

Biochemical and Physiological Mechanisms

Catalyzes the transfer of PtdIns and phosphatidylcholine between membranes.

Host species

Mouse

Specificity

PITPNB

Immunogen

Recombinant full length protein of human PITPNB.

Positive Control

WB: HEK293, A431 and RAW 264.7 cell lysates. IHC: Human kidney carcinoma, endometrium adenocarcinoma tissues.

Conjugation

Unconjugated

Grade

ExactAb™, High Performance, KO Validation

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

See COA

Storage and Shipping

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: PITPNB Mouse mAb (Ab121957)

PITPNB Mouse mAb (Ab121957) - Western Blot
All lanes: PITPNB Mouse mAb (Ab121957) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 31 kDa
Observed band size: 32 kDa
Exposure time: 64.0 s

Western Blot: PITPNB Mouse mAb (Ab121957)

PITPNB Mouse mAb (Ab121957) - Western Blot
Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells and PITPNB-Knockout HeLa cells were separated by SDS - PAGE and immunoblotted with PITPNB Mouse mAb (Ab121957) (1/500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody as a loading control.

IHC: PITPNB Mouse mAb (Ab121957)

PITPNB Mouse mAb (Ab121957) - IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using PITPNB Mouse mAb (Ab121957) . (Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 min, Ab121957) (1/2000).

IHC: PITPNB Mouse mAb (Ab121957)

PITPNB Mouse mAb (Ab121957) - IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using PITPNB Mouse mAb (Ab121957) . (Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 min, Ab121957) (1/2000).

Application

• Application: Dilution info
• WB:

  1/500-1/2000;

• IHC:

  1/2000;

Associated Targets(Human)

PITPNB Tbio Phosphatidylinositol transfer protein beta isoform (0 Activities)

Discover Target: PITPNB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Frequently Asked Questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does KO Validation mean?

KO Validation indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PITPNB Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.