BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at 2-8°C,Protected from light Ships Wet ice Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Proline (Pro) is a cyclic α-imino acid with a neutral nature and an isoelectric point of 6.30. It exhibits greater solubility in water than any other amino acid, with approximately 162g soluble in 100g of water at 25°C. It is hygroscopic and does not readily form crystals, possessing a sweet taste. When heated with ninhydrin solution, proline produces a yellow compound. Once incorporated into a peptide chain, it can undergo hydroxylation to form 4-hydroxyproline, a crucial component of animal collagen. Hydroxyproline is also present in various plant proteins, particularly in relation to cell wall formation. Under normal conditions, proline levels are relatively low; however, under stress conditions such as drought, heat, cold, freezing, or salinity, a significant accumulation of proline often occurs. The accumulation index is correlated with plant stress tolerance, and proline has wide applications in clinical settings, biomaterials, and industry. The detection principle of this kit is based on the reaction between free proline in a sulfosalicylic acid solution and acidic ninhydrin upon heating, which generates a stable red product. The absorbance at 520 nm is measured using a microplate reader, and within a certain concentration range, the intensity of the color is proportional to the proline concentration. This kit is primarily used for determining the free proline content in plant tissues and is intended for research purposes only. It is not suitable for clinical diagnosis or other applications.
Comprehensive hazard, handling, storage, and regulatory compliance document.
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